ArticleIUCrJ2020
Continuous flexibility analysis of SARS-CoV-2 spike prefusion structures.
Article in IUCrJ, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed.
- Flavonoids as Structural Probes Reveal Conformationally Dependent Ligand Recognition in the SARS-CoV-2 JN.1 Spike Protein.International journal of molecular sciences · 2026Article
- SARS-CoV-2 Spike Protein XBB.1.5 Mutations Altered Four Conserved Antigenic Determinants.International journal of molecular sciences · 2026Article
- Point cloud deformation modeling for particle selection following cryo-EM 2D classification.BMC bioinformatics · 2026Article
- Cleaved versus Uncleaved: How furin cleavage reshapes the conformational landscape of SARS-CoV-2 spike.Protein science : a publication of the Protein Society · 2025Article
- Exploring the Intrinsic Structural Plasticity and Conformational Dynamics of Human Beta Coronavirus Spike Glycoproteins.Journal of chemical information and modeling · 2025Article
- Allosteric Control and Glycan Shielding Adaptations in the SARS-CoV-2 Spike from Early to Peak Virulence.bioRxiv : the preprint server for biology · 2025Article
- Article
- KsgA facilitates ribosomal small subunit maturation by proofreading a key structural lesion.Nature structural & molecular biology · 2023Article
- 3DFlex: determining structure and motion of flexible proteins from cryo-EM.Nature methods · 2023Article
- Methods for Cryo-EM Single Particle Reconstruction of Macromolecules Having Continuous Heterogeneity.Journal of molecular biology · 2023Review
- Article
- Estimating conformational landscapes from Cryo-EM particles by 3D Zernike polynomials.Nature communications · 2023Article
- Protective neutralizing epitopes in SARS-CoV-2.Immunological reviews · 2022Review
- Article
- The structural role of SARS-CoV-2 genetic background in the emergence and success of spike mutations: The case of the spike A222V mutation.PLoS pathogens · 2022Article
- Evolution of the SARS-CoV-2 proteome in three dimensions (3D) during the first 6 months of the COVID-19 pandemic.Proteins · 2022Article
- Article
- Insights on the mutational landscape of the SARS-CoV-2 Omicron variant receptor-binding domain.Cell reports. Medicine · 2022Article
- Multifaceted membrane binding head of the SARS-CoV-2 spike protein.Current research in structural biology · 2022Article
- Functional binding dynamics relevant to the evolution of zoonotic spillovers in endemic and emergentJournal of biomolecular structure & dynamics · 2022Article
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Authors and funding
18 authors.
Funding
Abstract
Using a new consensus-based image-processing approach together with principal component analysis, the flexibility and conformational dynamics of the SARS-CoV-2 spike in the prefusion state have been analysed. These studies revealed concerted motions involving the receptor-binding domain (RBD), N-terminal domain, and subdomains 1 and 2 around the previously characterized 1-RBD-up state, which have been modeled as elastic deformations. It is shown that in this data set there are not well defined, stable spike conformations, but virtually a continuum of states. An ensemble map was obtained with minimum bias, from which the extremes of the change along the direction of maximal variance were modeled by flexible fitting. The results provide a warning of the potential image-processing classification instability of these complicated data sets, which has a direct impact on the interpretability of the results.
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