ArticleAmerican journal of human biology : the official journal of the Human Biology Council2021
Dried blood spot collection, sample quality, and fieldwork conditions: Structural validations for conversion into standard values.
Article in American journal of human biology : the official journal of the Human Biology Council, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
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Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it, 24 citations in OpenAlex.
- Dried Blood Spots to Assess Cardiovascular-Kidney-Metabolic Health.Journal of the American Heart Association · 2025Pooled it
- Results of the AiM, PLan, and act on LIFestYles (AMPLIFY) Weight Loss Intervention Among Survivors of Obesity-Related Cancers: A Randomized Clinical Trial.Journal of the National Comprehensive Cancer Network : JNCCN · 2026Trial
- Diabetes care performance across Europe: a cascade of care approach accounting for population diversity in 10 countries.BMC public health · 2026Article
- Do men and women define health differently? A cross-national study of gender differences in self-rated health.Frontiers in public health · 2026Article
- Investigating psychosocial factors and systemic inflammation using dried blood spots: a scoping review.Social psychiatry and psychiatric epidemiology · 2025Article
- Prevalence, awareness, treatment, and control of diabetes in India: a nationally representative survey of adults aged 45 years and older.The Lancet. Global health · 2025Article
- Undiagnosed diabetes mellitus among older adults: a harmonized cross-country analysis of prevalence, regional disparities and risk factors in 11 European countries and Israel.BMC public health · 2025Article
- High unawareness of kidney dysfunction in European older adults and the importance of early detection through comorbidities.PloS one · 2025Article
- Identification of different blood concentration of domestic cat (Open veterinary journal · 2024Article
- Harmonization of four biomarkers across nine nationally representative studies of older persons.American journal of human biology : the official journal of the Human Biology Council · 2024Article
- Dried blood spot based biomarkers in the Health and Retirement Study: 2006 to 2016.American journal of human biology : the official journal of the Human Biology Council · 2024Article
- A global metabolomics minefield: Confounding effects of preanalytical factors when studying rare disorders.Analytical science advances · 2023Article
- Article
- The Long Arm of childhood hypothesis and systematic low-grade inflammation: Evidence from parental education of older European adults.SSM - population health · 2023Article
- Effects of spot size on biomarker levels of field-collected dried blood spots: A new algorithm for exact dried blood spot size measurement.American journal of human biology : the official journal of the Human Biology Council · 2022Article
- Article
- Validation and application of a needs-based segmentation tool for cross-country comparisons.Health services research · 2021Article
- Article
- Assessment of circulating apoE4 levels from dried blood spot samples in a large survey setting.Alzheimer's & dementia (Amsterdam, Netherlands)Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
Abstract
objectivesSHARE, a pan-European panel study in 27 European countries and Israel, has collected dried blood spot (DBS) samples from approximately 27 000 respondents in 13 countries. We aim to obtain factors to convert analyte values between DBS and venous blood samples (VBS) taking account of adverse fieldwork conditions such as small spot size, high temperature and humidity, short drying time and long shipment times.
methodsWe obtained VBS and DBS from a set of 20 donors in a laboratory setting, and treated the DBS in a systematic and controlled fashion simulating SHARE fieldwork conditions. We used the 3420 outcomes to estimate from DBS analyte values the values that we would have obtained had it been feasible to collect and analyze the donors' venous blood samples.
resultsThe influence of field conditions and sample quality on DBS analyte values is significant and differs among assays. Varying spot size is the main challenge and affects all markers except HbA1c. Smaller spots lead to overly high measured levels. A missing desiccant is detrimental for all markers except CRP and tHb. The temperature to which the samples are exposed plays a significant role for HDL and CysC, while too brief a drying time affects CRP and CysC. Lab-based adjustment formulae only accounting for the differences between re-liquefied DBS and venous blood do not address these fieldwork conditions.
conclusionsBy simulating adverse fieldwork conditions in the lab, we were able to validate DBS collected under such conditions and established conversion formulae with high prediction accuracy.
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