ArticleInternational journal of reproductive biomedicine2020
Improvement in the epigenetic modification and development competence in PCOS mice oocytes by hydro-alcoholic extract of
Article in International journal of reproductive biomedicine, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 13 citations in OpenAlex.
- The Impact of Oxidative Stress Imbalance on Ovarian Function and Its Mechanisms.International journal of general medicine · 2026Review
- The latest reports and treatment methods on polycystic ovary syndrome.Annals of medicine · 2024Review
- Insights on the NF-κB system in polycystic ovary syndrome, attractive therapeutic targets.Molecular and cellular biochemistry · 2024Review
- Comprehensive and updated review on anti-oxidant effects ofIranian journal of basic medical sciences · 2024Review
- A Review of Clinical and Preclinical Studies on the Therapeutic Potential of Black Seeds (Journal of pharmacopuncture · 2023Review
- Phytochemicals: Alternative for Infertility Treatment and Associated Conditions.Oxidative medicine and cellular longevity · 2023Review
- Alternative treatment of polycystic ovary syndrome: pre-clinical and clinical basis for using plant-based drugs.Frontiers in endocrinology · 2023Review
- Female infertility and herbal medicine: An overview of the new findings.Food science & nutrition · 2021Review
- The LINC00477/miR-128 axis promotes the progression of polycystic ovary syndrome by regulating ovarian granulosa cell proliferation and apoptosis.Reproductive biology and endocrinology : RB&E · 2021Article
- Thymoquinone alleviates the adverse effects of cyclophosphamide on oogenesis and in vitro fertilization (IVF) in NMRI mice.Avicenna journal of phytomedicineArticle
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4 authors at 2 institutions in 1 country.
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Abstract
backgroundNigella Sativa (NS) and its active component, thymoquinone, have beneficial protective effects on experimental animal models of polycystic ovary syndrome (PCOS) and different human diseases.
objectiveThe present study aimed to investigate the effects of NS hydro-alcoholic extract (NSE) on the oocyte quality of PCOS mice during in vitro maturation. MATERIALS AND
methodsFor induction of PCOS, 40 prepubertal 21-days old female B6D2F1 mice (18-22 g body weight) received subcutaneous dehydroepiandrosterone daily. After validation of the model, germinal vesicle-stage oocytes of superovulated mice were collected and placed in the culture medium containing different concentrations (0, 1, 50, and 100 μg/ml) of NSE. For the measurement of developmental competency, some mature oocytes were fertilized with epididymal spermatozoa. Other mature oocytes were assessed for oxidative stress. Also, some mRNA expression levels involved in oocyte maturation and epigenetic modification were evaluated.
resultsThe 50 μg/ml NSE treated group showed significantly higher r ates o f maturation, f ertilization, and blastocyst formation in comparison with both control and PCOS groups. A high level of glutathione concentration and glutathione peroxidase mRNA expression, besides a low level of reactive oxygen species content all, were observed in oocytes treated with 50 μg/ml NSE, indicating the modification of oxidative statue. Furthermore, the oocytes in the 50 μg/ml-treated group showed an upregulation of mRNA expression in epigenetic-related genes (
conclusionThe results of this study indicated that 50 μg/ml NSE improves oocyte maturation, oxidative statues and epigenetic modifications. These may be the all reasons for the developmental competency in the control and PCOS mice oocytes.
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