ArticleBiotechnology for biofuels2020
Engineering endogenous ABC transporter with improving ATP supply and membrane flexibility enhances the secretion of β-carotene in
Article in Biotechnology for biofuels, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 86 citations in OpenAlex.
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- Research progress on metabolic engineering for efficient squalene synthesis in yeast cell factories.Biotechnology letters · 2026Review
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- Metabolic Engineering of Komagataella Phaffii for de Novo Synthesis of Retinol From Methanol.Biotechnology journal · 2026Article
- Synthetic Biology of Sclareol: From the Plant Biosynthetic Pathway to Engineered Microbial and Photosynthetic Chassis.Biotechnology journal · 2026Review
- High yield strategies for triterpenoid biosynthesis in cell factories.Journal of microbiology (Seoul, Korea) · 2026Review
- Enhancing delta-tocotrienol production inSynthetic and systems biotechnology · 2026Article
- Production of β-carotene from methanol via rDNA-targeted multi-gene integration and selection with increasing antibiotic concentrations in Komagataella phaffii.World journal of microbiology & biotechnology · 2026Article
- Synthesis of Amines for Active Pharmaceutical Ingredients Using the Whole-Cell Factory Saccharomyces Cerevisae.Chembiochem : a European journal of chemical biology · 2026Review
- A multi-way SMILES-based hypergraph inference network for metabolic model reconstruction.Communications biology · 2026Article
- Enhancement of growth and zeaxanthin accumulation via gene expression and metabolic pathways modulation in Chromochloris zofingiensis mutant CZ-Z12.Biotechnology for biofuels and bioproducts · 2026Article
- Push-pull-block framework for lycopene biosynthesis: a comprehensive review of metabolic engineering strategies inFrontiers in bioengineering and biotechnology · 2026Review
- Advances in the sustainable biosynthesis of valuable terpenoid flavor compounds and precursors in micro-organisms.Biotechnology for biofuels and bioproducts · 2025Review
- Sustainable β-carotene production by engineered S. cerevisiae using sucrose and agricultural by-products.Bioresources and bioprocessing · 2025Article
- Enhanced vitamin A production by engineering transporters, ATP and precursor supply.Biodesign research · 2025Article
- Engineering microorganisms for enhanced tolerance to toxic end-products and intermediates.FEMS microbiology reviews · 2025Review
- A transcriptome-guided genetic engineering strategy to balance cell growth with astaxanthin production inFrontiers in microbiology · 2025Article
- Review
- Transportation engineering for enhanced production of plant natural products in microbial cell factories.Synthetic and systems biotechnology · 2024Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundProduct toxicity is one of the bottlenecks for microbial production of biofuels, and transporter-mediated biofuel secretion offers a promising strategy to solve this problem. As a robust microbial host for industrial-scale production of biofuels,
resultsSubstrate inducibility is a prominent characteristic of most endogenous transporters. Through comparative proteomic analysis and transcriptional confirmation, we identified five potential ABC transporters (Pdr5p, Pdr10p, Snq2p, Yor1p, and Yol075cp) for β-carotene efflux. The accumulation of β-carotene also affects cell physiology in various aspects, including energy metabolism, mitochondrial translation, lipid metabolism, ergosterol biosynthetic process, and cell wall synthesis. Here, we adopted an inducible GAL promoter to overexpress candidate transporters and enhanced the secretion and intracellular production of β-carotene, in which Snq2p showed the best performance (a 4.04-fold and a 1.33-fold increase compared with its parental strain YBX-01, respectively). To further promote efflux capacity, two strategies of increasing ATP supply and improving membrane fluidity were following adopted. A 5.80-fold increase of β-carotene secretion and a 1.71-fold increase of the intracellular β-carotene production were consequently achieved in the engineered strain YBX-20 compared with the parental strain YBX-01.
conclusionsOverall, our results showcase that engineering endogenous plasma membrane ABC transporters is a promising approach for hydrophobic product efflux in
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