Evidence map›Paper›PMID 33061237›Full record

ArticleVeterinary world2020

Histological classification and expression of markers of canine mast cell tumors.

V S Cruz, J C A Borges, L L Nepomuceno, P A M Gonçalves, Y C L Prado, C Bianchi, M C S Fioravanti, E G Araújo

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Veterinary world, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.9field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 9 citations in OpenAlex.

  1. Review
  2. Article
  3. Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

V S CruzMulti-User Laboratory for the Evaluation of Molecules Cells and Tissues, Veterinary and Zootechnical School of the Federal University of Goiás, Campus Samambaia, Avenida Esperança, Goiânia, GO 74690-900, Brazil.
J C A BorgesMulti-User Laboratory for the Evaluation of Molecules Cells and Tissues, Veterinary and Zootechnical School of the Federal University of Goiás, Campus Samambaia, Avenida Esperança, Goiânia, GO 74690-900, Brazil.
L L NepomucenoMulti-User Laboratory for the Evaluation of Molecules Cells and Tissues, Veterinary and Zootechnical School of the Federal University of Goiás, Campus Samambaia, Avenida Esperança, Goiânia, GO 74690-900, Brazil.
P A M GonçalvesMulti-User Laboratory for the Evaluation of Molecules Cells and Tissues, Veterinary and Zootechnical School of the Federal University of Goiás, Campus Samambaia, Avenida Esperança, Goiânia, GO 74690-900, Brazil.
Y C L PradoDepartment of Veterinary Medicine of the University Center Nossa Senhora do Patrocínio, Pc Antônio Vieira Tavares, 73, Salto - SP, 13320-219, Brazil.
C BianchiDepartment of Experimental Medicine of the University of Mogi das Cruzes, Av. Dr. Cândido X. de Almeida e Souza, 200 - Centro Cívico, Mogi das Cruzes - SP, 08780-911, Brazil.
M C S FioravantiMulti-User Laboratory for the Evaluation of Molecules Cells and Tissues, Veterinary and Zootechnical School of the Federal University of Goiás, Campus Samambaia, Avenida Esperança, Goiânia, GO 74690-900, Brazil.
E G AraújoMulti-User Laboratory for the Evaluation of Molecules Cells and Tissues, Veterinary and Zootechnical School of the Federal University of Goiás, Campus Samambaia, Avenida Esperança, Goiânia, GO 74690-900, Brazil.
Universidade Federal de Goiás · BRCentro Universitário Nossa Senhora do Patrocínio · BRUniversidade de Mogi das Cruzes · BR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

BACKGROUND AND

aimMast cell tumors (MCTs) are malignant neoplasms that are common in dogs. Their biological behavior is variable and unpredictable. The aim of the present study was to analyze the histological classification and expression of markers of canine MCTs. MATERIALS AND

methodsThirty samples of canine MCTs were graded according to the histological classification methods of Patnaik and those of Kiupel. The expression of phosphoprotein 53 (p53) and c-kit proteins was quantified by immunohistochemistry using image processing software, ImageJ - a public domain computer program, developed at the National Institutes of Health.

resultsIt was possible to determine the grade of 100% of the samples. According to Patnaik's classification, 20.00% of the samples were Grade 1, 43.30% were Grade 2, and 36.70% were Grade 3. According to Kiupel's classification, 56.67% of the samples were of high intensity and 43.33% were of low intensity. Grade 1 tumors had the highest expression of p53 and c-kit, and Grade 2 had the lowest expression. The results showed that it is necessary to perform both histological grading methods. The classification into high and low intensity may provide more consistent results than the three-level grading system. However, a smaller number of categories, although it facilitates the classification, may not be sufficient for the prognosis.

conclusionQuantitative evaluation of p-53 and c-kit expression is a useful tool to increase the accuracy of the analysis and to aid in choosing the treatment method for canine MCTs. Histological grading should be combined with other diagnostic methods.

Indexed as

Bismarck brownhematoxylin and eosinImageJround cell tumortoluidine blue

Identifiers

PMID33061237
PMCPMC7522949
OpenAlexW3079070307

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.