ArticleThe ISME journal2021
Experimentally-validated correlation analysis reveals new anaerobic methane oxidation partnerships with consortium-level heterogeneity in diazotrophy.
Article in The ISME journal, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Carbon monoxide oxidation expands the known metabolic capacity in anaerobic methanotrophic consortia.Nature communications · 2026Article
- Anaerobic oxidation of methane supports a minimal microbial community in a subsurface biofilm at Ginsburg mud volcano.ISME communications · 2026Article
- Beyond Methane Oxidation: The Protein Landscape of ANME-2a Reveals an Integrated System for Diazotrophy and Membrane Fortification.Environmental microbiology reports · 2025Article
- Marine Cold Seep ANME-2/SRB Consortia Produce Their Lipid Biomass From Inorganic Carbon.Environmental microbiology · 2025Article
- Methane cycling microorganisms drive seasonal variation of methane emission in mangrove ecosystems.Environmental microbiome · 2025Article
- Methanotrophic Flexibility of 'Ca. Methanoperedens' and Its Interactions With Sulphate-Reducing Bacteria in the Sediment of Meromictic Lake Cadagno.Environmental microbiology · 2025Article
- Identification of key steps in the evolution of anaerobic methanotrophy inScience advances · 2025Article
- CABO-16S-a Combined Archaea, Bacteria, Organelle 16S rRNA database framework for amplicon analysis of prokaryotes and eukaryotes in environmental samples.NAR genomics and bioinformatics · 2025Article
- Microbial communities and metabolic functions vary with spatial heterogeneity in cold-seep carbonates.ISME communications · 2025Article
- Article
- A vast repertoire of secondary metabolites potentially influences community dynamics and biogeochemical processes in cold seeps.Science advances · 2024Article
- Global abundance patterns, diversity, and ecology of Patescibacteria in wastewater treatment plants.Microbiome · 2024Article
- Exploring the interplay between running exercises, microbial diversity, and tryptophan metabolism along the microbiota-gut-brain axis.Frontiers in microbiology · 2024Article
- Detection, isolation and characterization of phage-host complexes using BONCAT and click chemistry.Frontiers in microbiology · 2024Article
- Phylogenetic diversity of functional genes in deep-sea cold seeps: a novel perspective on metagenomics.Microbiome · 2023Article
- Mycorrhizal C/N ratio determines plant-derived carbon and nitrogen allocation to symbiosis.Communications biology · 2023Article
- Physiological potential and evolutionary trajectories of syntrophic sulfate-reducing bacterial partners of anaerobic methanotrophic archaea.PLoS biology · 2023Article
- A Cristae-Like Microcompartment inmBio · 2022Article
- Phylogenetically and catabolically diverse diazotrophs reside in deep-sea cold seep sediments.Nature communications · 2022Article
- Microbial communities of Auka hydrothermal sediments shed light on vent biogeography and the evolutionary history of thermophily.The ISME journal · 2022Article
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Archaeal anaerobic methanotrophs ("ANME") and sulfate-reducing Deltaproteobacteria ("SRB") form symbiotic multicellular consortia capable of anaerobic methane oxidation (AOM), and in so doing modulate methane flux from marine sediments. The specificity with which ANME associate with particular SRB partners in situ, however, is poorly understood. To characterize partnership specificity in ANME-SRB consortia, we applied the correlation inference technique SparCC to 310 16S rRNA amplicon libraries prepared from Costa Rica seep sediment samples, uncovering a strong positive correlation between ANME-2b and members of a clade of Deltaproteobacteria we termed SEEP-SRB1g. We confirmed this association by examining 16S rRNA diversity in individual ANME-SRB consortia sorted using flow cytometry and by imaging ANME-SRB consortia with fluorescence in situ hybridization (FISH) microscopy using newly-designed probes targeting the SEEP-SRB1g clade. Analysis of genome bins belonging to SEEP-SRB1g revealed the presence of a complete nifHDK operon required for diazotrophy, unusual in published genomes of ANME-associated SRB. Active expression of nifH in SEEP-SRB1g within ANME-2b-SEEP-SRB1g consortia was then demonstrated by microscopy using hybridization chain reaction (HCR-) FISH targeting nifH transcripts and diazotrophic activity was documented by FISH-nanoSIMS experiments. NanoSIMS analysis of ANME-2b-SEEP-SRB1g consortia incubated with a headspace containing CH
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