ArticleScientific reports2020
Accurate and sensitive detection of Salmonella in foods by engineered bacteriophages.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 1 of them a synthesis that pooled it.
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Who cites it
16 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Pooled it
- Using Risk-Benefit Analysis and the Analytical Hierarchy Process to Decide on the Implementation of Rapid Salmonella Detection Methods in Large Poultry Industries.Risk analysis : an official publication of the Society for Risk Analysis · 2026Article
- Biosensing technologies for foodborne pathogen detection and healthcare: principles, emerging materials, and intelligent platforms.Mikrochimica acta · 2026Review
- Generating STEC-SpecificViruses · 2025Article
- Pentameric nanobodies serve as a capture agent and RANbodies function as immunoprobes for the sensitive detection of Salmonella typhimurium in immunoassays.Analytical and bioanalytical chemistry · 2025Article
- Bacteriophage-based bioassays: an expected paradigm shift in microbial diagnostics.Future microbiology · 2024Review
- Genomic and Proteomic Analysis of Six Vi01-like Phages Reveals Wide Host Range and Multiple Tail Spike Proteins.Viruses · 2024Article
- Development of a Replication-Deficient Bacteriophage Reporter Lacking an Essential Baseplate Wedge Subunit.Viruses · 2023Article
- Advances in detection methods for viable Salmonella spp.: current applications and challenges.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2023Review
- Tailoring the Host Range ofViruses · 2023Article
- Recent Advances in the Application of Bacteriophages against Common Foodborne Pathogens.Antibiotics (Basel, Switzerland) · 2022Review
- Bacteriophage-Based Detection ofViruses · 2022Article
- Isolation and engineering of a Listeria grayi bacteriophage.Scientific reports · 2021Article
- Evaluation of PhageDXFoods (Basel, Switzerland) · 2021Article
- Review
- Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Salmonella is a major causative agent of foodborne illness and rapid identification of this pathogen is essential to prevent disease. Currently most assays require high bacterial burdens or prolonged enrichment to achieve acceptable performance. A reduction in testing time without loss of sensitivity is critical to allow food processors to safely decrease product holding time. To meet this need, a method was developed to detect Salmonella using luciferase reporter bacteriophages. Bacteriophages were engineered to express NanoLuc, a novel optimized luciferase originating from the deep-sea shrimp Oplophorus gracilirostris. NanoLuc-expressing bacteriophages had a limit of detection of 10-100 CFU per mL in culture without enrichment. Luciferase reporters demonstrated a broad host range covering all Salmonella species with one reporter detecting 99.3% of 269 inclusivity strains. Cross-reactivity was limited and only observed with other members of the Enterobacteriaceae family. In food matrix studies, a cocktail of engineered bacteriophages accurately detected 1 CFU in either 25 g of ground turkey with a 7 h enrichment or 100 g of powdered infant formula with a 16 h enrichment. Use of the NanoLuc reporter assay described herein resulted in a considerable reduction in enrichment time without a loss of sensitivity.
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