ArticleScientific reports2020
In vitro plant regeneration and Agrobacterium-mediated genetic transformation of a carnivorous plant, Nepenthes mirabilis.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- High-yield RNA extraction from Nepenthes × ventrata pitchers by simple modification of the CTAB method.Biotechnology letters · 2026Article
- Improving Ramie Fiber: Current Progress and Future Directions in Molecular Breeding.Plants (Basel, Switzerland) · 2026Review
- Efficient in vitro regeneration and overcoming premature senescence of Chenopodium quinoa willd.Scientific reports · 2025Article
- Carnivorous Nepenthes pitcher plants combine common developmental processes to make a complex epidermal trapping surface.Annals of botany · 2025Article
- Nuclear integration of MYB36 and APX-1 genes impart heat tolerance in wheat.Functional & integrative genomics · 2024Article
- Simplified Method forMethods and protocols · 2024Article
- 9-Tricosene Containing Blend of Volatiles Produced by Serratia sp. NhPB1 Isolated from the Pitcher Plant Provide Plant Protection Against Pythium aphanidermatum.Applied biochemistry and biotechnology · 2023Article
- Biological Potential of Carnivorous Plants from Nepenthales.Molecules (Basel, Switzerland) · 2023Review
- Draft genome and transcriptome of Nepenthes mirabilis, a carnivorous plant in China.BMC genomic data · 2023Article
- Secondary Metabolites with Biomedical Applications from Plants of the Sarraceniaceae Family.International journal of molecular sciences · 2022Review
- In vitro regeneration and Agrobacterium-mediated genetic transformation of Dragon's Head plant (Lallemantia iberica).Scientific reports · 2022Article
- Genetic Basis of Carnivorous Leaf Development.Frontiers in plant science · 2021Review
- Article
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5 authors.
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Abstract
In nutrient-poor habitats, carnivorous plants have developed novel feeding strategies based on the capture and digestion of prey and the assimilation of prey-derived nutrients by specialized traps. The Nepenthes genus, comprising nearly 160 species, presents a remarkable pitcher-shaped trap, leading to great interest among biologists, but the species of this genus are listed as threatened. In this work, we developed a protocol for reproducing Nepenthes mirabilis through shoot regeneration from calli. The cultivation of stem segments of N. mirabilis on MS medium containing thidiazuron induced organogenic calli after 10 weeks. Subcultured calli exposed to 6-benzylaminopurine showed shoot regeneration in 3 weeks with considerable yields (143 shoots/g of calli). Excised shoots transferred to medium with indole-3-butyric acid allowed rooting in 4 weeks, and rooted plantlets had a 100% survival rate. Based on this method, we also developed an Agrobacterium-mediated genetic transformation protocol using calli as explants and ipt as a positive method of selection. Twelve weeks post infection, regenerated shoots were observed at the surface of calli. Their transgenic status was confirmed by PCR and RT-PCR. In conclusion, this study provides an efficient method for regenerating Nepenthes and the first protocol for its stable genetic transformation, a new tool for studying carnivory.
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