ArticleNature communications2020
21-nt phasiRNAs direct target mRNA cleavage in rice male germ cells.
Article in Nature communications, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed, 94 citations in OpenAlex.
- Multiple Argonaute complexes orchestrate epigenetic silencing in the Arabidopsis germline.Nature communications · 2026Article
- A phased small interfering RNA acts in an OsAGO2-dependent manner to mediate OsARF7 cleavage and promote male fertility in rice.Plant communications · 2026Article
- RNA regulation in plants.Science China. Life sciences · 2026Review
- Premeiotic chromatin states orchestrate gene expression during male gametogenesis in rice.Genome biology · 2026Article
- Augmented CENH3 loading is accompanied by transcriptional and epigenetic reprogramming at rice centromeres during meiosis.Genome biology · 2026Article
- Reproductive phasiRNAs are the piRNAs of plants.Trends in genetics : TIG · 2026Review
- Cascaded regulatory network composed of small RNAs involves in the symbiosis of Panax notoginseng and fungus Acremonium sp. D212.Scientific reports · 2026Article
- Pre-meiotic H1.1 degradation is essential for Arabidopsis gametogenesis.The EMBO journal · 2026Article
- Integrated analysis of transcriptome, sRNAome and degradome sequencing provides insights into bacterial wilt resistance in potato.BMC plant biology · 2025Article
- Spatiotemporal regulation of target mRNA cleavage by 21-nt phasiRNAs in maize anthers.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Advancements in hybrid rice production: improvements in male sterility and synthetic apomixis for sustainable agriculture.Plant biotechnology journal · 2025Review
- An organ-specific transcriptome atlas of Curcuma wenyujin: MicroRNAs, phasiRNAs, and metabolic pathways.The plant genome · 2025Article
- MicroRNA identification and expression analysis of wheat thermo-sensitive male sterile line BNS366 for fertility transformation.Frontiers in plant science · 2025Article
- From chromatin to crop: epigenetic innovations in bioenergy systems.Frontiers in plant science · 2025Review
- Heterochromatin in plant meiosis.Nucleus (Austin, Tex.) · 2024Review
- The biogenesis, regulation and functions of transitive siRNA in plants.Acta biochimica et biophysica Sinica · 2024Review
- Premeiotic 24-nt phasiRNAs are present in theProceedings of the National Academy of Sciences of the United States of America · 2024Article
- DNA methylation remodeling and the functional implication during male gametogenesis in rice.Genome biology · 2024Article
- Premeiotic 24-nt phasiRNAs are present in thebioRxiv : the preprint server for biology · 2024Article
- MicroRNA482/2118 is lineage-specifically involved in gibberellin signalling via the regulation of GID1 expression by targeting noncoding PHAS genes and subsequently instigated phasiRNAs.Plant biotechnology journal · 2024Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In grasses, phased small interfering RNAs (phasiRNAs), 21- or 24-nucleotide (nt) in length, are predominantly expressed in anthers and play a role in regulating male fertility. However, their targets and mode of action on the targets remain unknown. Here we profile phasiRNA expression in premeiotic and meiotic spikelets as well as in purified male meiocytes at early prophase I, tetrads and microspores in rice. We show that 21-nt phasiRNAs are most abundant in meiocytes at early prophase I while 24-nt phasiRNAs are more abundant in tetrads and microspores. By performing highly sensitive degradome sequencing, we find that 21-nt phasiRNAs direct target mRNA cleavage in male germ cells, especially in meiocytes at early prophase I. These targets include 435 protein-coding genes and 71 transposons that show an enrichment for carbohydrate biosynthetic and metabolic pathways. Our study provides strong evidence that 21-nt phasiRNAs act in a target-cleavage mode and may facilitate the progression of meiosis by fine-tuning carbohydrate biosynthesis and metabolism in male germ cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.