Evidence map›Paper›PMID 33060138›Full record

ArticleGenetics2020

Improving Transgenesis Efficiency and CRISPR-Associated Tools Through Codon Optimization and Native Intron Addition in

Ziduan Han, Wen-Sui Lo, James W Lightfoot, Hanh Witte, Shuai Sun, Ralf J Sommer

Open access · bronzeAbstract read
In one paragraph

Article in Genetics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.

0numbers the graph read from it
0cells of the map it votes in
40citing papers in PubMed
2.4field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

40 citing papers in PubMed, 65 citations in OpenAlex.

  1. Article
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  4. Evolution of sensory systems underlies the emergence of predatory feeding behaviors in nematodes.Proceedings of the National Academy of Sciences of the United States of America · 2026
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  8. Identity and functions of monoaminergic neurons in the predatory nematodebioRxiv : the preprint server for biology · 2025
    Article
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  11. Article
  12. Article
  13. High Nutritional Conditions Influence Feeding Plasticity in Pristionchus pacificus and Render Worms Non-Predatory.Journal of experimental zoology. Part B, Molecular and developmental evolution · 2025
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Ziduan Han *Max Planck Institute for Developmental Biology, Tuebingen 72076, Germany.ORCID 0000-0001-8341-0678
Wen-Sui Lo *Max Planck Institute for Developmental Biology, Tuebingen 72076, Germany.ORCID 0000-0002-2438-0015
James W LightfootMax Planck Institute for Developmental Biology, Tuebingen 72076, Germany.ORCID 0000-0002-5835-2135
Hanh WitteMax Planck Institute for Developmental Biology, Tuebingen 72076, Germany.
Shuai SunMax Planck Institute for Developmental Biology, Tuebingen 72076, Germany.
Ralf J SommerMax Planck Institute for Developmental Biology, Tuebingen 72076, Germany ralf.sommer@tuebingen.mpg.de.ORCID 0000-0003-1503-7749
Max Planck Institute for Developmental Biology · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A lack of appropriate molecular tools is one obstacle that prevents in-depth mechanistic studies in many organisms. Transgenesis, clustered regularly interspaced short palindromic repeats (CRISPR)-associated engineering, and related tools are fundamental in the modern life sciences, but their applications are still limited to a few model organisms. In the phylum Nematoda, transgenesis can only be performed in a handful of species other than

Indexed as

Codon UsageCRISPR-Cas SystemsTransgenesAnimalsGene EditingIntronsRhabditidaC. eleganscodon usage biasCRISPR editingintron-mediated enhancementnematodesparasitic nematodesP. pacificustransgenesis

Identifiers

PMID33060138
PMCPMC7768246
OpenAlexW3092922863

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.