ArticleBMC pulmonary medicine2020
Optimal specimen type for accurate diagnosis of infectious peripheral pulmonary lesions by mNGS.
Article in BMC pulmonary medicine, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 1 synthesis or guideline pooled it, 40 citations in OpenAlex.
- Clinical and diagnostic values of metagenomic next-generation sequencing for infection in hematology patients: a systematic review and meta-analysis.BMC infectious diseases · 2024Pooled it
- Diagnosis of pleural aspergillosis caused by Aspergillus infection via metagenomic next-generation sequencing from a patient with unexplained pleural effusion: a case report.BMC pulmonary medicine · 2025Article
- Metagenomic next-generation sequencing for etiological diagnosis of an unexpected rabies case with unclear exposure history.BMC infectious diseases · 2025Article
- Neonatal microbiome dysbiosis decoded by mNGS: from mechanistic insights to precision interventions.Frontiers in cellular and infection microbiology · 2025Review
- A single-center, retrospective study of hospitalized patients with lower respiratory tract infections: clinical assessment of metagenomic next-generation sequencing and identification of risk factors in patients.Respiratory research · 2024Article
- Application of metagenomic next-generation sequencing in the clinical diagnosis of infectious diseases after allo-HSCT: a single-center analysis.BMC infectious diseases · 2024Article
- Joint application of metagenomic next-generation sequencing and histopathological examination for the diagnosis of pulmonary infectious disease.Microbiology spectrum · 2024Article
- Bronchoalveolar lavage fluid and lung biopsy tissue metagenomic next-generation sequencing in the diagnosis of pulmonary cryptococcosis.Frontiers in cellular and infection microbiology · 2024Article
- The role of metagenomic next-generation sequencing in diagnosing and managing post-kidney transplantation infections.Frontiers in cellular and infection microbiology · 2024Review
- Analysis of the utility of transbronchial lung biopsy culture under endobronchial ultrasonography with a guide-sheath.Scientific reports · 2023Article
- Metagenomic next-generation sequencing for rapid detection of pulmonary infection in patients with acquired immunodeficiency syndrome.Annals of clinical microbiology and antimicrobials · 2023Article
- Clinical Evaluation of Metagenomic Next-Generation Sequencing for the detection of pathogens in BALF in severe community acquired pneumonia.Italian journal of pediatrics · 2023Article
- Validation of metagenomic next-generation sequencing of bronchoalveolar lavage fluid for diagnosis of suspected pulmonary infections in patients with systemic autoimmune rheumatic diseases receiving immunosuppressant therapy.Frontiers in medicine · 2023Article
- High specificity of metagenomic next-generation sequencing using protected bronchial brushing sample in diagnosing pneumonia in children.Frontiers in cellular and infection microbiology · 2023Article
- Clinical application of amplification-based versus amplification-free metagenomic next-generation sequencing test in infectious diseases.Frontiers in cellular and infection microbiology · 2023Article
- A case report of diagnosis of cat-scratch disease using metagenomic next-generation sequencing.Frontiers in cellular and infection microbiology · 2023Article
- Evaluation of Metagenomic and Targeted Next-Generation Sequencing Workflows for Detection of Respiratory Pathogens from Bronchoalveolar Lavage Fluid Specimens.Journal of clinical microbiology · 2022Article
- Value of metagenomic next-generation sequencing in children with severe infectious diseases.Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics · 2022Article
- Metagenomic next-generation sequencing may assist diagnosis of cat-scratch disease.Frontiers in cellular and infection microbiology · 2022Article
- A Paired Comparison of Plasma and Bronchoalveolar Lavage Fluid for Metagenomic Next-Generation Sequencing in Critically Ill Patients with Suspected Severe Pneumonia.Infection and drug resistance · 2022Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 1 country.
Funding
Abstract
backgroundReports on the application of metagenomic next-generation sequencing (mNGS) to the diagnosis of peripheral pulmonary lesions (PPLs) are scarce. There have been no studies investigating the optimal specimen type for mNGS.
methodsWe used mNGS to detect pathogens in matched transbronchial lung biopsy (TBLB), bronchoalveolar lavage fluid (BALF), and bronchial needle brushing (BB) specimens from 39 patients suspected of having infectious PPLs. We explored differences in microbial composition and diagnostic accuracy of mNGS for the 3 specimen types.
resultsmNGS was more sensitive than conventional culture for detection of bacteria and fungi in TBLB, BALF, and BB specimens, with no difference in the sensitivity of mNGS across the different specimen types. mNGS showed higher sensitivity for fungi or uncategorized pulmonary pathogens in TBLB+BALF+BB compared to TBLB but not BALF or BB specimens. There were no significant differences between the 3 specimen types in the relative abundance of pathogens, or between TBLB and BB specimens in the relative abundance of 6 common lower respiratory tract commensals.
conclusionsmNGS has a higher sensitivity than the conventional culture method for detecting pathogens in TBLB, BALF, or BB specimens. mNGS of BB samples is a less invasive alternative to TBLB for the diagnosis of infectious PPLs.
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