ArticleMolecular ecology resources2021
msGBS: A new high-throughput approach to quantify the relative species abundance in root samples of multispecies plant communities.
Article in Molecular ecology resources, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Genetic Diversity Unveiled: Cost-Effective Methods for Grassland Species.Molecular ecology resources · 2026Article
- Comparing the precision of two digital PCR applications for copy number comparisons in protists.Scientific reports · 2025Article
- DNA Barcoding and Metabarcoding Protocols for Species Identification.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Plants, pollinators and their interactions under global ecological change: The role of pollen DNA metabarcoding.Molecular ecology · 2023Article
- Root litter decomposition is suppressed in species mixtures and in the presence of living roots.The Journal of ecology · 2023Article
- Genotyping by sequencing for estimating relative abundances of diatom taxa in mock communities.BMC ecology and evolution · 2023Article
- Optimisation of root traits to provide enhanced ecosystem services in agricultural systems: A focus on cover crops.Plant, cell & environment · 2022Review
- msGBS: A new high-throughput approach to quantify the relative species abundance in root samples of multispecies plant communities.Molecular ecology resources · 2021Article
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Authors and funding
8 authors.
Funding
Abstract
Plant interactions are as important belowground as aboveground. Belowground plant interactions are however inherently difficult to quantify, as roots of different species are difficult to disentangle. Although for a couple of decades molecular techniques have been successfully applied to quantify root abundance, root identification and quantification in multispecies plant communities remains particularly challenging. Here we present a novel methodology, multispecies genotyping by sequencing (msGBS), as a next step to tackle this challenge. First, a multispecies meta-reference database containing thousands of gDNA clusters per species is created from GBS derived High Throughput Sequencing (HTS) reads. Second, GBS derived HTS reads from multispecies root samples are mapped to this meta-reference which, after a filter procedure to increase the taxonomic resolution, allows the parallel quantification of multiple species. The msGBS signal of 111 mock-mixture root samples, with up to 8 plant species per sample, was used to calculate the within-species abundance. Optional subsequent calibration yielded the across-species abundance. The within- and across-species abundances highly correlated (R
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