Evidence map›Paper›PMID 33058333›Full record

ArticleChembiochem : a European journal of chemical biology2021

Stabilizing AqdC, a Pseudomonas Quinolone Signal-Cleaving Dioxygenase from Mycobacteria, by FRESCO-Based Protein Engineering.

Sandra C Wullich, Hein J Wijma, Dick B Janssen, Susanne Fetzner

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Chembiochem : a European journal of chemical biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.3field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 7 citations in OpenAlex.

  1. Review
  2. Protein Stability: Enhancement and Measurement.Methods in molecular biology (Clifton, N.J.) · 2023
    Article
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 2 countries.

Sandra C WullichInstitut für Molekulare Mikrobiologie und Biotechnologie, WWU Münster, Corrensstraße 3, 48149,  Münster, Germany.ORCID 0000-0003-1887-0736
Hein J WijmaDepartment of Biochemistry Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG, Groningen (The, Netherlands.ORCID 0000-0003-0891-6972
Dick B JanssenDepartment of Biochemistry Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG, Groningen (The, Netherlands.ORCID 0000-0002-0834-2043
Susanne FetznerInstitut für Molekulare Mikrobiologie und Biotechnologie, WWU Münster, Corrensstraße 3, 48149,  Münster, Germany.ORCID 0000-0002-7228-1871
University of Groningen · NLUniversity of Münster · DE

Funding

Deutsche Forschungsgemeinschaft FE 383/25-1
6 · The paper itself

Abstract

The mycobacterial PQS dioxygenase AqdC, a cofactor-less protein with an α/β-hydrolase fold, inactivates the virulence-associated quorum-sensing signal molecule 2-heptyl-3-hydroxy-4(1H)-quinolone (PQS) produced by the opportunistic pathogen Pseudomonas aeruginosa and is therefore a potential anti-virulence tool. We have used computational library design to predict stabilizing amino acid replacements in AqdC. While 57 out of 91 tested single substitutions throughout the protein led to stabilization, as judged by increases in

Indexed as

Bacterial ProteinsDioxygenasesGene Expression Regulation, BacterialMycobacteriumProtein EngineeringPseudomonas aeruginosaQuinolonesQuorum SensingSignal Transduction2-heptyl-3-hydroxy-4-quinoloneBacterial ProteinsDioxygenasesQuinolonesalpha/beta hydrolase foldprotein engineeringsignal moleculestructure-activity relationshipsthermostability

Identifiers

PMID33058333
PMCPMC7894191
OpenAlexW3093426892

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.