ArticleScientific reports2020
The impact of handling technique and handling frequency on laboratory mouse welfare is sex-specific.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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Who cites it
50 citing papers in PubMed, 74 citations in OpenAlex.
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- Enhancing Rodent Welfare, Cost Savings, and Efficiency: Implementation and Review of Data-Driven Improvements.Journal of the American Association for Laboratory Animal Science : JAALAS · 2026Review
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- Collagen binding adhesin restricts Staphylococcus aureus skin infection.Nature communications · 2026Article
- Peripartum sertraline impacts maternal neurobehavioral and neurodegenerative mechanisms in pregnant and postpartum mice.Molecular psychiatry · 2025Article
- Social Housing of Male CD-1 Mice (Mus musculus) in the Toxicological Setting: A 28-Day Social Compatibility Study of Male Mice following Oral Gavage of Theophylline.Journal of the American Association for Laboratory Animal Science : JAALAS · 2025Article
- Breathing Rate as a Marker for Noise-Induced Stress in Guinea Pigs.Brain sciences · 2025Article
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- Morphological features of the domestic house cricket (Acheta domesticus) for translational aging studies.GeroScience · 2025Article
- Training Mice to Perform Attentional Set-Shifting Under Head Restraint.Bio-protocol · 2025Article
- A novel miR-4536-3p inhibition ameliorates Alzheimer's disease by reducing Aβ accumulation and tau phosphorylation.Alzheimer's research & therapy · 2025Article
- Lack of evidence for a consistent differential impact of tail and tunnel handling on markers of welfare in laboratory mice.Scientific reports · 2025Article
- Menopause-induced 17β-estradiol and progesterone loss increases senescence markers, matrix disassembly and degeneration in mouse cartilage.Nature aging · 2025Article
- Narirutin treatment accelerates the process of diabetic wound repair by regulating phenotype switching of macrophages through affecting metabolic reprogramming.Frontiers in pharmacology · 2025Article
- The effects of handling on mouse behavior: cupped hands versus familiar or novel huts or tunnels.PloS one · 2025Article
- Sex differences in spontaneous behavior and cognition in mice using an automated behavior monitoring system.Physiology & behavior · 2024Article
- Effects of Nesting Material and Housing Parameters on Feed Wastage Behavior in Female Swiss Webster Mice.Journal of the American Association for Laboratory Animal Science : JAALAS · 2024Article
- Effects of Intermittent Harem Mating on Parturition-induced Stress and Success Rate of Weaning in C57BL/6JNarl Mice.Journal of the American Association for Laboratory Animal Science : JAALAS · 2024Article
- Influence of Diet on Reproducible Corticosterone Levels in a Mouse Model of Maternal Separation with Early Weaning.Life (Basel, Switzerland) · 2024Article
Corrections and comments
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Authors and funding
8 authors at 5 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Handling is a well-known source of stress to laboratory animals and can affect variability of results and even compromise animal welfare. The conventional tail handling in mice has been shown to induce aversion and anxiety-like behaviour. Recent findings demonstrate that the use of alternative handling techniques, e.g. tunnel handling, can mitigate negative handling-induced effects. Here, we show that technique and frequency of handling influence affective behaviour and stress hormone release of subjects in a sex-dependent manner. While frequent tail handling led to a reduction of wellbeing-associated burrowing and increased despair-like behaviour in male mice, females seemed unaffected. Instead, they displayed a stress response to a low handling frequency, which was not detectable in males. This could suggest that in terms of refinement, the impact in handling could differ between the sexes. Independently from this observation, both sexes preferred to interact with the tunnel. Mice generally explored the tunnel more often than the tail-handling hands of the experimenter and showed more positively rated approaches, e.g. touching or climbing, and at the same time, less defensive burrowing, indicating a strong preference for the tunnel.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.