ArticleNature communications2020
Fermentative production of Vitamin E tocotrienols in Saccharomyces cerevisiae under cold-shock-triggered temperature control.
Article in Nature communications, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 84 citations in OpenAlex.
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- Comparative neuroprotective and antioxidant roles of vitamin E isoforms: a review.Journal of clinical biochemistry and nutrition · 2026Review
- A non-classical biosynthetic route enables tocopherol synthesis in bacteria.Nature metabolism · 2026Article
- Advances in the heterologous biosynthesis of QS saponins: a review.Microbial cell factories · 2026Review
- TARDBP Mediates the MAP3K11/SLC3A2/GPX4 Axis in Alzheimer's Disease Rats by Enhancing KRAS mRNA Stability.Journal of cellular and molecular medicine · 2026Article
- Metabolic Engineering ofJournal of fungi (Basel, Switzerland) · 2026Article
- A century of vitamin E research: The innovative journey from basic biology to synthetic bio-manufacturing.Journal of integrative plant biology · 2026Review
- Balancing Cell Growth and Product Synthesis for Efficient Microbial Cell Factories.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- A Review of the Progress in the Microbial Biosynthesis of Prenylated Aromatic Compounds.Molecules (Basel, Switzerland) · 2025Review
- Combined metabolic and enzymatic engineering forSynthetic and systems biotechnology · 2025Article
- Mechanisms and Strategies for Engineering Oxidative Stress Resistance inChem & bio engineering · 2025Review
- The Response Characteristics of OneJournal of fungi (Basel, Switzerland) · 2025Article
- Composition, Influencing Factors, and Effects on Host Nutrient Metabolism of Fungi in Gastrointestinal Tract of Monogastric Animals.Animals : an open access journal from MDPI · 2025Review
- Rhizosphere domestication enhances root colonization and plant growth promotion performance ofFrontiers in microbiology · 2025Article
- Intein-mediated temperature control for complete biosynthesis of sanguinarine and its halogenated derivatives in yeast.Nature communications · 2024Article
- Hypolipidemic Effect of Rice Bran Oil Extract Tocotrienol in High-Fat Diet-Induced Hyperlipidemia Zebrafish (Danio Rerio) Induced by High-Fat Diet.International journal of molecular sciences · 2024Article
- A novel ferroptosis inhibitor, Thonningianin A, improves Alzheimer's disease by activating GPX4.Theranostics · 2024Article
- Reshaping the yeast galactose regulon via GPCR signaling cascade.Cell reports methods · 2023Article
- Advances in the dynamic control of metabolic pathways inEngineering microbiology · 2023Review
- Vitamins as regulators of calcium-containing kidney stones - new perspectives on the role of the gut microbiome.Nature reviews. Urology · 2023Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The diverse physiological functions of tocotrienols have listed them as valuable supplementations to α-tocopherol-dominated Vitamin E products. To make tocotrienols more readily available, tocotrienols-producing S. cerevisiae has been constructed by combining the heterologous genes from photosynthetic organisms with the endogenous shikimate pathway and mevalonate pathway. After identification and elimination of metabolic bottlenecks and enhancement of precursors supply, the engineered yeast can produce tocotrienols at yield of up to 7.6 mg/g dry cell weight (DCW). In particular, proper truncation of the N-terminal transit peptide from the plant-sourced enzymes is crucial. To further solve the conflict between cell growth and tocotrienols accumulation so as to enable high-density fermentation, a cold-shock-triggered temperature control system is designed for efficient control of two-stage fermentation, leading to production of 320 mg/L tocotrienols. The success in high-density fermentation of tocotrienols by engineered yeast sheds light on the potential of fermentative production of vitamin E tocochromanols.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.