Evidence map›Paper›PMID 33054752›Full record

ArticleBMC cancer2020

YB-1 transferred by gastric cancer exosomes promotes angiogenesis via enhancing the expression of angiogenic factors in vascular endothelial cells.

Xiaoxia Xue, Jin Huang, Kai Yu, Xinyue Chen, Yini He, Dianjun Qi, Ying Wu

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC cancer, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.

0numbers the graph read from it
0cells of the map it votes in
38citing papers in PubMed
2.0field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

38 citing papers in PubMed, 48 citations in OpenAlex.

  1. Article
  2. Review
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  4. Article
  5. Article
  6. Functions and Clinical Applications of Exosomes in Gastric Cancer.International journal of biological sciences · 2025
    Review
  7. Review
  8. Review
  9. Article
  10. Article
  11. Article
  12. Review
  13. Review
  14. Review
  15. Review
  16. Article
  17. Review
  18. Article
  19. YB1 and its role in osteosarcoma: a review.Frontiers in oncology · 2024
    Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Xiaoxia XueScience Experiment Center, China Medical University, 77 Puhe Road, Shenyang North New Area, Liaoning, 110122, Shenyang, China.
Jin HuangDepartment of Radiation Therapy, The First Hospital, China Medical University, 518 Chuangxin Road, Hunnan District, Shenyang, 110167, Liaoning, China.
Kai YuDepartment of General Practice, The First Hospital, China Medical University, 155 South Nanjing Street, Heping District, Shenyang, 110001, Liaoning, China.
Xinyue ChenDepartment of General Practice, The First Hospital, China Medical University, 155 South Nanjing Street, Heping District, Shenyang, 110001, Liaoning, China.
Yini HeDepartment of General Practice, The First Hospital, China Medical University, 155 South Nanjing Street, Heping District, Shenyang, 110001, Liaoning, China.
Dianjun QiDepartment of General Practice, The First Hospital, China Medical University, 155 South Nanjing Street, Heping District, Shenyang, 110001, Liaoning, China.
Ying WuDepartment of General Practice, The First Hospital, China Medical University, 155 South Nanjing Street, Heping District, Shenyang, 110001, Liaoning, China. wuying@cmu.edu.cn.
China Medical University · CNFirst Hospital of China Medical University · CN

Funding

National Natural Science Foundation of China 81201801
6 · The paper itself

Abstract

backgroundAngiogenesis is important for the progression of gastric cancer (GC). Y-box binding protein 1 (YB-1) predicts advanced disease and indicates neovasculature formation in GC tissues, while the related mechanisms remain elusive. Exosomes mediate intercellular communications via transferring various molecules including proteins, lipids, mRNAs, and microRNAs, while the cargos of GC exosomes and the related mechanisms in GC angiogenesis were rarely reported except for several microRNAs.

methodsIn this study, human umbilical vein endothelial cells (HUVECs) were, respectively, treated by the exosomes isolated from the YB-1 transfected and the control SGC-7901 cells (SGC-7901-OE-Exo and SGC-7901-NC-Exo), and their apoptosis, proliferation, migration, invasion, and angiogenesis were, sequentially, compared. The levels of angiogenic factors including VEGF, Ang-1, MMP-9 and IL-8 in the exosome-treated HUVECs and the GC-derived exosomes were, separately, detected using PCR and Western blotting as well as RNA sequencing assays.

resultsWe observed the consistent level of YB-1 in the exosomes and their originated GC cells, and the internalization of exosomes into HUVECs. Comparing with SGC-7901-NC-Exo, SGC-7901-OE-Exo significantly inhibited the apoptosis but promoted the proliferation, migration, invasion, and angiogenesis of HUVECs, within which the increased mRNA and protein levels of VEGF, Ang-1, MMP-9 and IL-8 were demonstrated. Meanwhile, mRNA levels of VEGF, Ang-1, MMP-9 and IL-8 showed no significant difference between SGC-7901-NC-Exo and SGC-7901-OE-Exo, although statistically higher mRNA of YB-1 was detected in the SGC-7901-OE-Exo.

conclusionsOur findings illustrate YB-1 as the key component of exosome to promote GC angiogenesis by upregulating specific angiogenic factors in the exosome-treated endothelial cells but not in the exosomes themselves.

Indexed as

Cell ProliferationEndothelial CellsExosomesHumansNeovascularization, PhysiologicStomach NeoplasmsY-Box-Binding Protein 1Y-Box-Binding Protein 1YBX1 protein, humanAng-1AngiogenesisExosomeGastric cancerIL-8MMP-9VEGFYB-1

Identifiers

PMID33054752
PMCPMC7557103
OpenAlexW3092739245

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.