ArticlePathogens (Basel, Switzerland)2020
Screening of Fish Cell Lines for Piscine Orthoreovirus-1 (PRV-1) Amplification: Identification of the Non-Supportive PRV-1 Invitrome.
Article in Pathogens (Basel, Switzerland), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 29 citations in OpenAlex.
- Identification and characterization of a novel orthoreovirus from American eels,Microbiology spectrum · 2026Article
- Improve your success with fish cell lines-small things that matter.In vitro cellular & developmental biology. Animal · 2026Review
- Development of a Novel Virus-Like Particle-Based Vaccine Against PRV-1 Suitable for DIVA Strategies.Viruses · 2025Article
- Toward Effective Vaccines Against Piscine Orthoreovirus: Challenges and Current Strategies.Viruses · 2025Review
- Article
- Comparative Analysis of PRV-1 in Atlantic Salmon and PRV-3 in Coho Salmon: Host-Specific Immune Responses and Apoptosis in Red Blood Cells.Microorganisms · 2025Article
- Beating Cardiac Cell Cultures From Different Developmental Stages of Rainbow Trout as a Novel Approach for Replication of Cardiac Fish Viruses.Journal of fish diseases · 2025Article
- Establishment and characterization of novel spontaneously immortalized larval cell lines from sablefish Anoplopoma fimbria.In vitro cellular & developmental biology. Animal · 2025Article
- Analyses and Insights into Genetic Reassortment and Natural Selection as Key Drivers ofViruses · 2024Article
- Fish cell line: depositories, web resources and future applications.Cytotechnology · 2024Review
- Transcriptomics of early responses to purifiedFrontiers in immunology · 2024Article
- Derivation and characterization of new cell line from intestine of turbot (Scophthalmus maximus).In vitro cellular & developmental biology. Animal · 2023Article
- Sleeping With the Enemy? The Current Knowledge of Piscine Orthoreovirus (PRV) Immune Response Elicited to Counteract Infection.Frontiers in immunology · 2022Review
- Pan-Piscine Orthoreovirus (PRV) Detection Using Reverse Transcription Quantitative PCR.Pathogens (Basel, Switzerland) · 2021Article
- Establishment of a new fish cell line from the brain of humpback grouper (Cromileptes altivelis) and its application in toxicology and bacterial susceptibility.Fish physiology and biochemistry · 2021Article
- Article
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Authors and funding
10 authors at 5 institutions in 1 country.
Funding
Abstract
Piscine reovirus (PRV) is the causative agent of heart and skeletal muscle inflammation (HSMI), which is detrimental to Atlantic Salmon (AS) aquaculture, but so far has not been cultivatable, which impedes studying the disease and developing a vaccine. Homogenates of head kidney and red blood cells (RBC) from AS in which PRV-1 had been detected were applied to fish cell lines. The cell lines were from embryos, and from brain, blood, fin, gill, gonads, gut, heart, kidney, liver, skin, and spleen, and had the shapes of endothelial, epithelial, fibroblast, and macrophage cells. Most cell lines were derived from the Neopterygii subclass of fish, but one was from subclass Chondrostei. Cultures were examined by phase contrast microscopy for appearance, and by quantitative polymerase chain reaction (qPCR) for PRV-1 RNA amplification and for the capacity to transfer any changes to new cultures. No changes in appearance and Ct values were observed consistently or transferable to new cultures. Therefore, 31 cell lines examined were unable to support PRV-1 amplification and are described as belonging to the non-supportive PRV-1 invitrome. However, these investigations and cell lines can contribute to understanding PRV-1 cellular and host tropism, and the interactions between virus-infected and bystander cells.
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Registered trials
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