ArticleCell reports2020
Phase-Separated Transcriptional Condensates Accelerate Target-Search Process Revealed by Live-Cell Single-Molecule Imaging.
Article in Cell reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 74 papers.
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Who cites it
74 citing papers in PubMed, 135 citations in OpenAlex.
- Cryo-EM structure, enzymatic activity and genome targeting of canonical PRC1.Nature structural & molecular biology · 2026Article
- Extracting anomalous diffusion parameters from multi-state ensembles of short single molecule trajectories.bioRxiv : the preprint server for biology · 2026Article
- H3K27me3 spreading organizes canonical PRC1 chromatin architecture to regulate developmental programs.Nature genetics · 2026Article
- Self-clustering of three CBX2 molecules drives PRC2 to promote facultative heterochromatinization of Polycomb target genes.Molecular cell · 2026Article
- Deregulated enhancer-promoter communication in cancer through altered nuclear architecture.International journal of cancer · 2026Review
- Development of ultrafast single fluorescent-molecule imaging and its application to unravel plasma membrane structure and function in live cells.Biophysics and physicobiology · 2026Article
- Chromatin Binding Regulates Phase Behavior and Morphology of Condensates Formed by Prion-like Domains.Journal of molecular biology · 2025Article
- Article
- Specificity in clustering of gene-specific transcription factors is encoded in the genome.Nucleic acids research · 2025Article
- Single-molecule tracking reveals the dynamic turnover of Ipl1 at the kinetochores inLife science alliance · 2025Article
- A disordered linker in the Polycomb protein Polyhomeotic tunes phase separation and oligomerization.Molecular cell · 2025Article
- Intrinsically disordered regions as facilitators of the transcription factor target search.Nature reviews. Genetics · 2025Review
- Phase Separation in Chromatin Organization and Human Diseases.International journal of molecular sciences · 2025Review
- Modular in vivo assembly of Arabidopsis FCA oligomers into condensates competent for RNA 3' processing.The EMBO journal · 2025Article
- Transcription dynamics and genome organization in the mammalian nucleus: Recent advances.Molecular cell · 2025Review
- Article
- Spatial Control over Reactions via Localized Transcription within Membraneless DNA Nanostar Droplets.Journal of the American Chemical Society · 2024Article
- Viral N protein hijacks deaminase-containing RNA granules to enhance SARS-CoV-2 mutagenesis.The EMBO journal · 2024Article
- Decoding the genomic landscape of chromatin-associated biomolecular condensates.Nature communications · 2024Article
- How Transcription Factor Clusters Shape the Transcriptional Landscape.Biomolecules · 2024Review
14 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Compartmentalization by liquid-liquid phase separation is implicated in transcription. It remains unclear whether and how transcriptional condensates accelerate the search of transcriptional regulatory factors for their target sites. Furthermore, the molecular mechanisms by which regulatory factors nucleate on chromatin to assemble transcriptional condensates remain incompletely understood. The CBX-PRC1 complexes compartmentalize key developmental regulators for repression through phase-separated condensates driven by the chromobox 2 (CBX2) protein. Here, by using live-cell single-molecule imaging, we show that CBX2 nucleates on chromatin independently of H3K27me3 and CBX-PRC1. The interactions between CBX2 and DNA are essential for nucleating CBX-PRC1 on chromatin to assemble condensates. The assembled condensates shorten 3D diffusion time and reduce trials for finding specific sites through revisiting the same or adjacent sites repetitively, thereby accelerating CBX2 in searching for target sites. Overall, our data suggest a generic mechanism by which transcriptional regulatory factors nucleate to assemble condensates that accelerate their target-search process.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.