Evidence map›Paper›PMID 33052483›Full record

ArticleBiotechnology letters2021

Effect of by-products from the dairy industry as alternative inducers of recombinant β-galactosidase expression.

Francielle Herrmann Mobayed, Juliane Carraro Nunes, Adriano Gennari, Bruna Coelho de Andrade, Matheus Loch Velvites Ferreira, Paolla Pauli, Gaby Renard, Jocelei Maria Chies, Giandra Volpato, Claucia Fernanda Volken de Souza

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Article in Biotechnology letters, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.7field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 9 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 4 institutions in 1 country.

Francielle Herrmann Mobayed *Laboratório de Biotecnologia de Alimentos, Universidade do Vale do Taquari - Univates, Av. Avelino Tallini, 171, ZC, Lajeado, RS, 95914-014, Brazil.
Juliane Carraro Nunes *Curso de Biotecnologia, Instituto Federal de Educação, Ciência e Tecnologia do Rio Grande do Sul - IFRS, Campus Porto Alegre, Porto Alegre, RS, Brazil.
Adriano GennariLaboratório de Biotecnologia de Alimentos, Universidade do Vale do Taquari - Univates, Av. Avelino Tallini, 171, ZC, Lajeado, RS, 95914-014, Brazil.
Bruna Coelho de AndradeLaboratório de Biotecnologia de Alimentos, Universidade do Vale do Taquari - Univates, Av. Avelino Tallini, 171, ZC, Lajeado, RS, 95914-014, Brazil.
Matheus Loch Velvites FerreiraCurso de Biotecnologia, Instituto Federal de Educação, Ciência e Tecnologia do Rio Grande do Sul - IFRS, Campus Porto Alegre, Porto Alegre, RS, Brazil.
Paolla PauliCurso de Biotecnologia, Instituto Federal de Educação, Ciência e Tecnologia do Rio Grande do Sul - IFRS, Campus Porto Alegre, Porto Alegre, RS, Brazil.
Gaby RenardCentro de Pesquisa em Biologia Molecular e Funcional, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, RS, Brazil.
Jocelei Maria ChiesQuatro G Pesquisa & Desenvolvimento Ltda, Porto Alegre, RS, Brazil.
Giandra VolpatoCurso de Biotecnologia, Instituto Federal de Educação, Ciência e Tecnologia do Rio Grande do Sul - IFRS, Campus Porto Alegre, Porto Alegre, RS, Brazil.
Claucia Fernanda Volken de SouzaLaboratório de Biotecnologia de Alimentos, Universidade do Vale do Taquari - Univates, Av. Avelino Tallini, 171, ZC, Lajeado, RS, 95914-014, Brazil. claucia@univates.br.ORCID http://orcid.org/0000-0001-7708-3762
Instituto Federal de Educação, Ciência e Tecnologia do Rio Grande do Sul · BRUnivates · BRFaculdade de Desenvolvimento do Rio Grande do Sul · BRPontifícia Universidade Católica do Rio Grande do Sul · BR

Funding

Conselho Nacional de Desenvolvimento Científico e Tecnológico 311655/2017-3Conselho Nacional de Desenvolvimento Científico e Tecnológico 315225/2018-1Conselho Nacional de Desenvolvimento Científico e Tecnológico 420506/2016-0
6 · The paper itself

Abstract

objectiveThe aim of the present study was to evaluate the efficiency of lactose derived from cheese whey and cheese whey permeate as inducer of recombinant Kluyveromyces sp. β-galactosidase enzyme produced in Escherichia coli. Two E. coli strains, BL21(DE3) and Rosetta (DE3), were used in order to produce the recombinant enzyme. Samples were evaluated for enzyme activity, total protein content, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis after induction with isopropyl-β-D-1-thiogalactoside (IPTG) (0.05 and 1 mM) and lactose, cheese whey, and cheese whey permeate solutions (1, 10, and 20 g/L lactose) at shake-flask cultivation, and whey permeate solution (10 g/L lactose) at bioreactor scale.

resultsThe highest specific activities obtained with IPTG as inducer (0.05 mM) after 9 h of induction, were 23 and 33 U/mg

conclusionsThe induction with cheese whey permeate was more efficient for recombinant β-galactosidase expression than the other inducers tested, and thus, represents an alternative form to reduce costs in recombinant protein production.

Indexed as

beta-GalactosidaseFungal ProteinsLactoseRecombinant ProteinsBioreactorsCheeseCulture MediaDairyingEscherichia coliKluyveromycesWheybeta-GalactosidaseCulture MediaFungal ProteinsLactoseRecombinant ProteinsCheese wheyCheese whey permeateEscherichia coliLactose

Identifiers

PMID33052483
OpenAlexW3092496030

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.