ReviewCurrent opinion in environmental science & health2020
Surveillance of SARS-CoV-2 RNA in wastewater: Methods optimisation and quality control are crucial for generating reliable public health information.
Review in Current opinion in environmental science & health, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 97 papers, 2 of them syntheses that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
97 citing papers in PubMed, 2 syntheses or guidelines pooled it.
- Factors influencing SARS-CoV-2 RNA concentrations in wastewater up to the sampling stage: A systematic review.The Science of the total environment · 2022Pooled it
- Systematic review of wastewater surveillance of antimicrobial resistance in human populations.Environment international · 2022Pooled it
- Detection of Respiratory Viruses in Wastewater Samples Using Commercial Multiplex PCR Assays.Viruses · 2026Article
- Assess the Variability and Robustness of an Aluminum-Based Adsorption-Precipitation Method for Virus Detection in Wastewater Samples.Microorganisms · 2025Article
- Advances in Wastewater-Based Epidemiology for Pandemic Surveillance: Methodological Frameworks and Future Perspectives.Microorganisms · 2025Review
- Performance of skimmed milk flocculation and real-time polymerase chain reaction for the concentration, detection, and quantification of Taenia solium in urban wastewater from an endemic region of the Peruvian central highlands.The Science of the total environment · 2025Article
- Article
- A suite of ddPCR assays targeting microbial pathogens for improved management of shellfish aquaculture.Applied and environmental microbiology · 2025Article
- The Impact of the Variability of RT-qPCR Standard Curves on Reliable Viral Detection in Wastewater Surveillance.Microorganisms · 2025Article
- Optimized Aluminum Hydroxide Adsorption-Precipitation for Improved Viral Detection in Wastewater.International journal of environmental research and public health · 2025Article
- Wastewater Surveillance for SARS-CoV-2 in Northern Italy: An Evaluation of Three Different Gene Targets.Microorganisms · 2025Article
- Epidemiological model can forecast COVID-19 outbreaks from wastewater-based surveillance in rural communities.Water research · 2025Article
- The effects of RT-qPCR standards on reproducibility and comparability in monitoring SARS-CoV-2 levels in wastewater.Scientific reports · 2024Article
- Sewage surveillance of SARS-CoV-2 in Tokyo (May 2020-June 2022): method using high-throughput, fully-automated platform.Virusdisease · 2024Article
- Public health use and lessons learned from a statewide SARS-CoV-2 wastewater monitoring program (MiNET).Heliyon · 2024Article
- SARS-CoV-2 viral titer measurements in Ontario, Canada wastewaters throughout the COVID-19 pandemic.Scientific data · 2024Article
- Wastewater-based surveillance as a tool for public health action: SARS-CoV-2 and beyond.Clinical microbiology reviews · 2024Review
- Article
- Wastewater-based reproduction numbers and projections of COVID-19 cases in three areas in Japan, November 2021 to December 2022.Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2024Article
- Wastewater-based epidemiology: deriving a SARS-CoV-2 data validation method to assess data quality and to improve trend recognition.Frontiers in public health · 2024Article
37 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
14 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Monitoring for SARS-CoV-2 RNA in wastewater through the process of wastewater-based epidemiology (WBE) provides an additional surveillance tool, contributing to community-based screening and prevention efforts as these measurements have preceded disease cases in some instances. Numerous detections of SARS-CoV-2 RNA have been reported globally using various methods, demonstrating the technical feasibility of routine monitoring. However, in order to reliably interpret data produced from these efforts for informing public health interventions, additional quality control information and standardization in sampling design, sample processing, and data interpretation and reporting is needed. This review summarizes published studies of SARS-CoV-2 RNA detection in wastewater as well as available information regarding concentration, extraction, and detection methods. The review highlights areas for potential standardization including considerations related to sampling timing and frequency relative to peak fecal loading times; inclusion of appropriate information on sample volume collected; sample collection points; transport and storage conditions; sample concentration and processing; RNA extraction process and performance; effective volumes; PCR inhibition; process controls throughout sample collection and processing; PCR standard curve performance; and recovery efficiency testing. Researchers are recommended to follow the Minimum Information for Publication of Quantitative Real-Time PCR (MIQE) guidelines. Adhering to these recommendations will enable robust interpretation of wastewater monitoring results and improved inferences regarding the relationship between monitoring results and disease cases.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.