ArticleeLife2020
Label-free imaging of immune cell dynamics in the living retina using adaptive optics.
Article in eLife, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
41 citing papers in PubMed.
- Characterization of Leukocyte Crawling in the Living Human Retina Using Adaptive Optics Scanning Light Ophthalmoscopy.Investigative ophthalmology & visual science · 2026Article
- Multi-orientation non-confocal retinal imaging in an AOSLO using a Dove prism.Biomedical optics express · 2026Article
- Sensitivity Enhancement of Dynamic Full-Field Optical Coherence Tomography Using Ratio-Free Detection and Partial-Field Illumination for Retinal Organoid Imaging.Bioengineering (Basel, Switzerland) · 2026Article
- Perivascular Macrophages Are Associated With Permissive Sites of Immune Cell Extravasation in Retinal Venules.Investigative ophthalmology & visual science · 2026Article
- Cellular-resolution OCT reveals layer-specific retinal mosaics and ganglion cell degeneration in mouse retinaNeurophotonics · 2026Article
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- BEHAV3D Tumor Profiler to map heterogeneous cancer cell behavior in the tumor microenvironment.eLife · 2025Article
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- Photoreceptor loss does not recruit neutrophils despite strong microglial activation.bioRxiv : the preprint server for biology · 2025Article
- Towards noninvasive white blood cell count.Biomedical optics express · 2025Article
- Quantification of optical lensing by cellular structures in the living human eye.Biomedical optics express · 2025Article
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- Estimation of biological aging based on T-cell differentiation trajectories: emerging and future avenues.Frontiers in aging · 2025Article
- Redefining our vision: an updated guide to the ocular immune system.Nature reviews. Immunology · 2024Review
- Improved tracking of corneal immune cell dynamics usingBiomedical optics express · 2024Article
- Intraocular Immune Response in Human Uveitis: Time to Look Beyond Animal Models.American journal of ophthalmology · 2024Review
- In Vivo Visualization of Intravascular Patrolling Immune Cells in the Primate Eye.Investigative ophthalmology & visual science · 2024Article
- Hyalocytes-guardians of the vitreoretinal interface.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2024Review
- Retinal perivascular macrophages regulate immune cell infiltration during neuroinflammation in mouse models of ocular disease.The Journal of clinical investigation · 2024Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
Our recent work characterized the movement of single blood cells within the retinal vasculature (Joseph et al. 2019) using adaptive optics ophthalmoscopy. Here, we apply this technique to the context of acute inflammation and discover both infiltrating and tissue-resident immune cells to be visible without any labeling in the living mouse retina using near-infrared light alone. Intravital imaging of immune cells can be negatively impacted by surgical manipulation, exogenous dyes, transgenic manipulation and phototoxicity. These confounds are now overcome, using phase contrast and time-lapse videography to reveal the dynamic behavior of myeloid cells as they interact, extravasate and survey the mouse retina. Cellular motility and differential vascular responses were measured noninvasively and in vivo across hours to months at the same retinal location, from initiation to the resolution of inflammation. As comparable systems are already available for clinical research, this approach could be readily translated to human application.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.