ArticleeLife2020
3D visualization of macromolecule synthesis.
Article in eLife, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
11 citing papers in PubMed, 16 citations in OpenAlex.
- A characterization of axolotl digit regeneration: conserved mechanisms, divergent patterning, and a critical role for hedgehog signaling.NPJ Regenerative medicine · 2026Article
- Best Practices to Promote Data Utility and Reuse by the Non-Traditional Model Organism Community.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Usability of deep learning pipelines for 3D nuclei identification with Stardist and Cellpose.Cells & development · 2022Article
- Post-Transcriptional Control of mRNA Metabolism and Protein Secretion: The Third Level of Regulation within the NF-κB System.Biomedicines · 2022Review
- Gene and transgenics nomenclature for the laboratory axolotl-Ambystoma mexicanum.Developmental dynamics : an official publication of the American Association of Anatomists · 2022Article
- Local mechanical stimuli correlate with tissue growth in axolotl salamander joint morphogenesis.Proceedings. Biological sciences · 2022Article
- A constitutively expressed fluorescent ubiquitination-based cell-cycle indicator (FUCCI) in axolotls for studying tissue regeneration.Development (Cambridge, England) · 2022Article
- Wnt Signaling Coordinates the Expression of Limb Patterning Genes During Axolotl Forelimb Development and Regeneration.Frontiers in cell and developmental biology · 2022Article
- Toward whole tissue imaging of axolotl regeneration.Developmental dynamics : an official publication of the American Association of Anatomists · 2021Review
- Salamander Insights Into Ageing and Rejuvenation.Frontiers in cell and developmental biology · 2021Article
- 3D visualization of macromolecule synthesis.eLife · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 3 countries.
Funding
Abstract
Measuring nascent macromolecular synthesis in vivo is key to understanding how cells and tissues progress through development and respond to external cues. Here we perform in vivo injection of alkyne- or azide-modified analogs of thymidine, uridine, methionine, and glucosamine to label nascent synthesis of DNA, RNA, protein, and glycosylation. Three-dimensional volumetric imaging of nascent macromolecule synthesis was performed in axolotl salamander tissue using whole-mount click chemistry-based fluorescent staining followed by light sheet fluorescent microscopy. We also developed an image processing pipeline for segmentation and classification of morphological regions of interest and individual cells, and we apply this pipeline to the regenerating humerus. We demonstrate our approach is sensitive to biological perturbations by measuring changes in DNA synthesis after limb denervation. This method provides a powerful means to quantitatively interrogate macromolecule synthesis in heterogenous tissues at the organ, cellular, and molecular levels of organization.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.