ArticleChembiochem : a European journal of chemical biology2021
Challenges of Using Expansion Microscopy for Super-resolved Imaging of Cellular Organelles.
Article in Chembiochem : a European journal of chemical biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 52 citations in OpenAlex.
- Resolving endogenous protein organization in cells with nanometer resolution.Nature communications · 2026Article
- Visualisation of peroxisomes: a journey through seven decades.Histochemistry and cell biology · 2026Review
- HAK-actin, a U-ExM-compatible probe to image the actin cytoskeleton.Cell reports methods · 2026Article
- Targeting organelle function in T cells for cancer immunotherapy.Nature reviews. Immunology · 2026Review
- Review of expansion microscopy combined with advanced imaging modalities.Journal of microscopy · 2026Review
- Single-Molecule Localization Expansion Nanoscopy via Spontaneously Blinking Fluorophores.Chemical & biomedical imaging · 2026Article
- Ultrastructural expansion microscopy reveals unexpected levels of glycosome heterogeneity in African trypanosomes.Journal of microscopy · 2026Article
- Optimized expansion microscopy reveals species-specific spindle microtubule organization inMolecular biology of the cell · 2025Article
- ExPOSE: a comprehensive toolkit to perform expansion microscopy in plant protoplast systems.The Plant journal : for cell and molecular biology · 2025Article
- One step 4× and 12× 3D-ExM enables robust super-resolution microscopy of nanoscale cellular structures.The Journal of cell biology · 2025Article
- Accelerated protein retention expansion microscopy using microwave radiation.Cell reports methods · 2024Article
- 3D-Aligner: advanced computational tool for correcting image distortion in expansion microscopy.Communications biology · 2024Article
- 3D-Aligner: An advanced computational tool designed to correct image distortion in expansion microscopy for precise 3D reconstitution and quantitative analysis.bioRxiv : the preprint server for biology · 2024Article
- One step 4x and 12x 3D-ExM: robust super-resolution microscopy in cell biology.bioRxiv : the preprint server for biology · 2024Article
- Accelerated protein retention expansion microscopy using microwave radiation.bioRxiv : the preprint server for biology · 2024Article
- Expanding boundaries - a cell biologist's guide to expansion microscopy.Journal of cell science · 2024Review
- Toward a comprehensive view of gene architecture during transcription.Current opinion in genetics & development · 2024Review
- Combining single-molecule and expansion microscopy in fission yeast to visualize protein structures at the nanostructural level.Open biology · 2024Article
- Enhanced synaptic protein visualization by multicolor super-resolution expansion microscopy.Neurophotonics · 2023Article
- Expansion microscopy reveals subdomains inLife science alliance · 2023Article
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
Expansion microscopy (ExM) has been successfully used to improve the spatial resolution when imaging tissues by optical microscopy. In ExM, proteins of a fixed sample are crosslinked to a swellable acrylamide gel, which expands when incubated in water. Therefore, ExM allows enlarged subcellular structures to be resolved that would otherwise be hidden to standard confocal microscopy. Herein, we aim to validate ExM for the study of peroxisomes, mitochondria, nuclei and the plasma membrane. Upon comparison of the expansion factors of these cellular compartments in HEK293 cells within the same gel, we found significant differences, of a factor of above 2, in expansion factors. For peroxisomes, the expansion factor differed even between peroxisomal membrane and matrix marker; this underlines the need for a thorough validation of expansion factors of this powerful technique. We further give an overview of possible quantification methods for the determination of expansion factors of intracellular organelles, and we highlight some potentials and challenges.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.