Evidence map›Paper›PMID 33048239›Full record

ArticlePediatric surgery international2020

miR-200 family expression during normal and abnormal lung development due to congenital diaphragmatic hernia at the later embryonic stage in the nitrofen rat model.

Drew Mulhall, Naghmeh Khoshgoo, Robin Visser, Barbara Iwasiow, Chelsea Day, Fuqin Zhu, Patrice Eastwood, Richard Keijzer

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Article in Pediatric surgery international, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.0field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 11 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 2 countries.

Drew MulhallBiology of Breathing Group, The Children's Hospital Research Institute of Manitoba, University of Manitoba, Winnipeg, MB, Canada.
Naghmeh KhoshgooBiology of Breathing Group, The Children's Hospital Research Institute of Manitoba, University of Manitoba, Winnipeg, MB, Canada.
Robin VisserBiology of Breathing Group, The Children's Hospital Research Institute of Manitoba, University of Manitoba, Winnipeg, MB, Canada.
Barbara IwasiowBiology of Breathing Group, The Children's Hospital Research Institute of Manitoba, University of Manitoba, Winnipeg, MB, Canada.
Chelsea DayBiology of Breathing Group, The Children's Hospital Research Institute of Manitoba, University of Manitoba, Winnipeg, MB, Canada.
Fuqin ZhuBiology of Breathing Group, The Children's Hospital Research Institute of Manitoba, University of Manitoba, Winnipeg, MB, Canada.
Patrice EastwoodDepartment of Development and Regeneration, Cluster Organ Systems, Faculty of Medicine, Katholieke Universiteit Leuven, Leuven, Belgium.
Richard KeijzerBiology of Breathing Group, The Children's Hospital Research Institute of Manitoba, University of Manitoba, Winnipeg, MB, Canada. richardkeijzer@gmail.com.ORCID http://orcid.org/0000-0002-0108-3157
Children's Hospital Research Institute of Manitoba · CAOrgan Recovery Systems (Belgium) · BEVanguard College · CA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

introductionCongenital diaphragmatic hernia (CDH) is a life-threatening disease associated with pulmonary hypoplasia. CDH occurs approximately 1 in every 2000-3000 live births, and the pathophysiology is unknown. MicroRNAs are short, non-coding RNAs that control gene expression through post-transcriptional regulation. Based on our previous work, we hypothesized that the miR-200 family is differentially expressed in normal and abnormal lung development. We aimed to examine the expression of the miR-200 family during normal and hypoplastic lung development due to CDH.

methodsWe performed reverse transcriptase polymerase chain reaction (RT-qPCR) and fluorescent in situ hybridization (FISH) to study the expression levels and distribution of the miR-200 family members on embryonic day 21 (E21) rat control and nitrofen-induced hypoplastic CDH lungs.

resultsRT-qPCR showed up-regulation of miR-200a in hypoplastic CDH lungs. FISH showed contrasting expression patterns for miR- 200a, miR-200c, and miR-429 between control and hypoplastic CDH lungs, while we could not detect miR-141 in control and hypoplastic CDH lungs.

conclusionWe demonstrate a specific expression pattern of miR-200 family members in hypoplastic CDH lungs different from control lungs. This study suggests that disruption of miR-200 family expression plays a role in the pathogenesis of pulmonary hypoplasia associated with CDH.

Indexed as

AnimalsDisease Models, AnimalFemaleGene ExpressionHernias, Diaphragmatic, CongenitalIn Situ Hybridization, FluorescenceLungMicroRNAsPhenyl EthersRatsRats, Sprague-DawleyReal-Time Polymerase Chain ReactionMicroRNAsMIRN200 microRNA, ratnitrofenPhenyl EthersCDHDevelopmental biologyEpigeneticsGeneticsLung developmentmicroRNA

Identifiers

PMID33048239
OpenAlexW3092441893

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.