ArticleLab on a chip2020
3× multiplexed detection of antibiotic resistant plasmids with single molecule sensitivity.
Article in Lab on a chip, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 20 citations in OpenAlex.
- Single molecule nanopore counting assay targeting small extracellular vesicle cargo for non-invasive monitoring of cerebral organoid development and health.Scientific reports · 2025Article
- Universal Nanopore Sensor for Amplification-Free and Label-Free Detection of Molecular Biomarkers.ACS sensors · 2025Article
- Portable microfluidic devices for monitoring antibiotic resistance genes in wastewater.Mikrochimica acta · 2024Review
- Label-free and amplification-free viral RNA quantification from primate biofluids using a trapping-assisted optofluidic nanopore platform.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Comparison of Illumination Methods for Flow-Through Optofluidic Biosensors.Micromachines · 2023Article
- Machine learning at the edge for AI-enabled multiplexed pathogen detection.Scientific reports · 2023Article
- A Novel Application of Spinning Disk Technology to Collect Plasma from Whole Blood Prior to Quantifying Plasma RNA.microPublication biology · 2023Article
- Fast custom wavelet analysis technique for single molecule detection and identification.Nature communications · 2022Article
- FPGA Integrated Optofluidic Biosensor for Real-Time Single Biomarker Analysis.IEEE photonics journal · 2022Article
- Advances in multiplex electrical and optical detection of biomarkers using microfluidic devices.Analytical and bioanalytical chemistry · 2022Review
- Recent advances in integrated solid-state nanopore sensors.Lab on a chip · 2021Review
- Free-Space Excitation of Optofluidic Devices for Pattern-Based Single Particle Detection.IEEE photonics technology letters : a publication of the IEEE Laser and Electro-optics Society · 2021Article
- Multilabel hybridization probes for sequence-specific detection of sepsis-related drug resistance genes in plasmids.Talanta open · 2021Article
- Article
- Optofluidic multiplex detection of single SARS-CoV-2 and influenza A antigens using a novel bright fluorescent probe assay.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Optofluidic Flow-Through Biosensor Sensitivity - Model and Experiment.Journal of lightwave technology : a joint IEEE/OSA publication · 2021Article
- Rapid and simple pressure-sensitive adhesive microdevice fabrication for sequence-specific capture and fluorescence detection of sepsis-related bacterial plasmid gene sequences.Analytical and bioanalytical chemistry · 2021Article
- Greatly Enhanced Single Particle Fluorescence Detection Using High Refractive Index Liquid-Core Waveguides.IEEE journal of selected topics in quantum electronics : a publication of the IEEE Lasers and Electro-optics SocietyArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 2 institutions in 1 country.
Funding
Abstract
Bacterial pathogens resistant to antibiotics have become a serious health threat. Those species which have developed resistance against multiple drugs such as the carbapenems, are more lethal as these are last line therapy antibiotics. Current diagnostic tests for these resistance traits are based on singleplex target amplification techniques which can be time consuming and prone to errors. Here, we demonstrate a chip based optofluidic system with single molecule sensitivity for amplification-free, multiplexed detection of plasmids with genes corresponding to antibiotic resistance, within one hour. Rotating disks and microfluidic chips with functionalized polymer monoliths provided the upstream sample preparation steps to selectively extract these plasmids from blood spiked with E. coli DH5α cells. Waveguide-based spatial multiplexing using a multi-mode interference waveguide on an optofluidic chip was used for parallel detection of three different carbapenem resistance genes. These results point the way towards rapid, amplification-free, multiplex analysis of antibiotic-resistant pathogens.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.