ArticleNPJ biofilms and microbiomes2020
Biological role of EPS from Pseudomonas syringae pv. syringae UMAF0158 extracellular matrix, focusing on a Psl-like polysaccharide.
Article in NPJ biofilms and microbiomes, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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22 citing papers in PubMed, 49 citations in OpenAlex.
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- High-resolution visualization of biofilm matrix development in space and time using fluorescent stains for cellulose.NPJ biofilms and microbiomes · 2026Article
- Root exudates protect rhizosphereApplied and environmental microbiology · 2025Article
- Functional characterization and biotechnological applications of exopolysaccharides produced by newly isolated Enterococcus hirae MLG3-25-1.International microbiology : the official journal of the Spanish Society for Microbiology · 2025Article
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- Combating biofilm-associated Klebsiella pneumoniae infections using a bovine microbial enzyme.NPJ biofilms and microbiomes · 2024Article
- Unveiling a novel exopolysaccharide produced by Pseudomonas alcaligenes Med1 isolated from a Chilean hot spring as biotechnological additive.Scientific reports · 2024Article
- Life on a leaf: the epiphyte to pathogen continuum and interplay in the phyllosphere.BMC biology · 2024Review
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- Exopolysaccharide Production and Precipitation Method as a Tool to Study Virulence Factors.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Role of extracellular matrix components in biofilm formation and adaptation ofFrontiers in microbiology · 2024Article
- FleQ, FleN and c-di-GMP coordinately regulate cellulose production inFrontiers in molecular biosciences · 2023Article
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- Comparative genomics and genomic diversity of Pseudomonas syringae clade 2b-a in Australia.BMC microbiology · 2022Article
- Microbe Related Chemical Signalling and Its Application in Agriculture.International journal of molecular sciences · 2022Review
- The periplasmic chaperone protein Psg_2795 contributes to the virulence of Pseudomonas savastanoi pv. glycinea: the causal agent of bacterial blight of soybean.Journal of microbiology (Seoul, Korea) · 2022Article
- Pseudomonas syringae addresses distinct environmental challenges during plant infection through the coordinated deployment of polysaccharides.Journal of experimental botany · 2022Article
- A regulatory network involving Rpo, Gac and Rsm for nitrogen-fixing biofilm formation by Pseudomonas stutzeri.NPJ biofilms and microbiomes · 2021Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pseudomonas syringae is a phytopathogenic model bacterium that is used worldwide to study plant-bacteria interactions and biofilm formation in association with a plant host. Within this species, the syringae pathovar is the most studied due to its wide host range, affecting both, woody and herbaceous plants. In particular, Pseudomonas syringae pv. syringae (Pss) has been previously described as the causal agent of bacterial apical necrosis on mango trees. Pss exhibits major epiphytic traits and virulence factors that improve its epiphytic survival and pathogenicity in mango trees. The cellulose exopolysaccharide has been described as a key component in the development of the biofilm lifestyle of the P. syringae pv. syringae UMAF0158 strain (PssUMAF0158). PssUMAF0158 contains two additional genomic regions that putatively encode for exopolysaccharides such as alginate and a Psl-like polysaccharide. To date, the Psl polysaccharide has only been studied in Pseudomonas aeruginosa, in which it plays an important role during biofilm development. However, its function in plant-associated bacteria is still unknown. To understand how these exopolysaccharides contribute to the biofilm matrix of PssUMAF0158, knockout mutants of genes encoding these putative exopolysaccharides were constructed. Flow-cell chamber experiments revealed that cellulose and the Psl-like polysaccharide constitute a basic scaffold for biofilm architecture in this bacterium. Curiously, the Psl-like polysaccharide of PssUMAF0158 plays a role in virulence similar to what has been described for cellulose. Finally, the impaired swarming motility of the Psl-like exopolysaccharide mutant suggests that this exopolysaccharide may play a role in the motility of PssUMAF0158 over the mango plant surface.
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