ArticleBMC veterinary research2020
Characterization of total adenosine deaminase activity (ADA) and its isoenzymes in saliva and serum in health and inflammatory conditions in four different species: an analytical and clinical validation pilot study.
Article in BMC veterinary research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 47 citations in OpenAlex.
- Investigating the Association Between Salivary Profiles, Production-Related Variables, and Daily Lying Time in Dairy Cows: A Pilot Study.Animals : an open access journal from MDPI · 2026Article
- The Salivary Microbiota-Host Nexus: Unraveling Opportunities for Non-Invasive Monitoring of Health and Productivity in Farm Animals.Animals : an open access journal from MDPI · 2026Review
- Targeting selenometabolites in the saliva of dairy cows: size-exclusion chromatography - inductively coupled plasma mass spectrometry-based characterization.Veterinary and animal science · 2026Article
- Thermal imaging: A potential tool for early detection of tail lesions in pigs?Animal welfare (South Mimms, England) · 2026Article
- Effect of Preweaning Socialization on Postweaning Biomarkers of Stress, Inflammation, Immunity and Metabolism in Saliva and Serum of Iberian Piglets.Animals : an open access journal from MDPI · 2025Article
- Fecal intestinal inflammatory biomarkers in piglets: analytical validation, homogeneity and stability studies.Veterinary and animal science · 2025Article
- Changes in saliva and serum analytes in domestic pigs and wild boar experimentally infected with African swine fever virus.Veterinary research · 2025Article
- Effect of age and disease on saliva and serum biomarkers of stress, inflammation, immunity, redox and general health status in pigs.Scientific reports · 2025Article
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- Uncovering genomic diversity and signatures of selection in red Angus × Chinese red steppe crossbred cattle population.Scientific reports · 2025Article
- Elevated levels of C-reactive protein and pig major acute phase protein in lame gestating sows.Frontiers in veterinary science · 2025Article
- Article
- Article
- Saliva as a Potential Source of Biomarkers in Cows with Metritis: A Pilot Study.Veterinary sciences · 2024Article
- New insights into swine dysentery: faecal shedding, macro and microscopic lesions and biomarkers in early and acute stages of Brachyspira hyodysenteriae infection.Porcine health management · 2024Article
- Salivary and serum haptoglobin, adenosine deaminase, and immunoglobulin G in growing pigs.Porcine health management · 2024Article
- Dietary fiber and weaning age affect stress and immune markers in saliva of sows and their offspring.Journal of animal science · 2024Article
- Salivary biomarkers as pioneering indicators for diagnosis and severity stratification of pediatric long COVID.Frontiers in cellular and infection microbiology · 2024Article
- Is adenosine deaminase (ADA) activity in saliva and serum a more accurate disease detection tool than traditional redox balance parameters in early-lactating dairy cows?Veterinary research communications · 2023Article
- Using integrated wildlife monitoring to prevent future pandemics through one health approach.One health (Amsterdam, Netherlands) · 2023Article
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
Abstract
backgroundMeasurement of adenosine deaminase (ADA) can provide information about cell-mediated immunity. This report's objective was to study the enzymatic activity of total ADA (tADA) and its isoenzymes ADA1 and ADA2 in canine, equine, porcine, and bovine serum and saliva and their changes in different inflammatory situations in each species. Besides, an automated method for ADA2 measurement was developed and validated.
resultstADA was present in serum and saliva of healthy animals of the four species. Erythro-9-(2-hydroxy-3-nonyl) adenine (EHNA) concentration of 0.47 mM was needed for ADA1 inhibition in canine and porcine samples (serum and saliva) and bovine saliva, whereas for equine saliva 0.94 mM was needed. ADA2 activity was not detected in bovine serum and was very low or absent in equine serum and bovine saliva. An automated procedure to measure ADA2 consisting of adding EHNA to a commercial reagent for tADA measurement provided repetitive (coefficients of variation < 8.8% in serum and < 10% in saliva) and accurate (linearity of serial sample dilutions with R
conclusionsThe activity of total ADA and its different isoenzymes, can be measured in serum and saliva of dogs, horses, pigs and cows by a simple and fast procedure described in this report. When measured in saliva, these analytes correlated with other biomarkers of inflammation and it could potentially be used as a biomarkers of inflammation and immune activation in the species of this study.
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