ArticleBMC oral health2020
Diagnostic biomarker candidates for pulpitis revealed by bioinformatics analysis of merged microarray gene expression datasets.
Article in BMC oral health, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 1 of them a synthesis that pooled it.
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Who cites it
24 citing papers in PubMed, 1 synthesis or guideline pooled it, 38 citations in OpenAlex.
- Meta-Analysis of Two Human RNA-seq Datasets to Determine Periodontitis Diagnostic Biomarkers and Drug Target Candidates.International journal of molecular sciences · 2022Pooled it
- Differential Expression Analysis of Key Inflammatory Mediators in Irreversible Pulpitis for Diagnostic Biomarkers.International dental journal · 2026Article
- Establishment of an MRI-Based Diagnostic Approach to Identify Critical Pulpal Inflammation Zones in a Rat Caries-Derived Pulpitis Model.International endodontic journal · 2026Article
- Pulpitis as a microbial disease: single-cell insights into host responses and diagnostic biomarkers for vital pulp therapy.Journal of oral microbiology · 2026Review
- Endodontic Inflammatory Disease: Systemic Consequences.Advances in experimental medicine and biology · 2026Review
- Objectively Diagnosing Pulpitis: Opportunities and Methodological Challenges in the Development of Point-of-Care Assays.International journal of molecular sciences · 2025Review
- Symptom correlation and spatial distribution of inflammatory mediators in pulpitis-A preliminary study.International endodontic journal · 2025Article
- Identification of pain-related long non-coding RNAs for pulpitis prediction.Clinical oral investigations · 2025Article
- Expert consensus on pulpotomy in the management of mature permanent teeth with pulpitis.International journal of oral science · 2025Review
- In silico prediction of differentially expressed genes and functionally grouped networks in patients with inflamed pulp for screening pulpitis biomarkers.European oral research · 2025Article
- Causal relationship, shared genes between rheumatoid arthritis and pulp and periapical disease: evidence from GWAS and transcriptome data.Frontiers in immunology · 2024Article
- Drug sensitivity testing during dental pulp-dentin complex repair: its effect on treatment outcome and prognosis.American journal of translational research · 2024Article
- Establishment and validation of the autophagy-related ceRNA network in irreversible pulpitis.BMC genomics · 2023Article
- Identification of the characteristics of infiltrating immune cells in pulpitis and its potential molecular regulation mechanism by bioinformatics method.BMC oral health · 2023Article
- Identification and Experimental Validation of LINC00582 Associated with B Cell Immune and Development of Pulpitis: Bioinformatics and In Vitro Analysis.Diagnostics (Basel, Switzerland) · 2023Article
- Biodentine but not MTA induce DSPP expression of dental pulp cells with different severity of LPS-induced inflammation.Clinical oral investigations · 2023Article
- Identification of key module and hub genes in pulpitis using weighted gene co-expression network analysis.BMC oral health · 2023Article
- Matrix Metalloproteinases in Dental and Periodontal Tissues and Their Current Inhibitors: Developmental, Degradational and Pathological Aspects.International journal of molecular sciences · 2022Review
- Long Non-coding RNA PVT1 is Involved in the Pathological Mechanism of Pulpitis by Regulating miR-128-3p.Oral health & preventive dentistry · 2022Article
- Role of LINC01133 in Osteogenic Differentiation of Dental Pulp Stem Cells by Targeting miR-199b-5p.Oral health & preventive dentistry · 2022Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
backgroundPulpitis is an inflammatory disease, the grade of which is classified according to the level of inflammation. Traditional methods of evaluating the status of dental pulp tissue in clinical practice have limitations. The rapid and accurate diagnosis of pulpitis is essential for determining the appropriate treatment. By integrating different datasets from the Gene Expression Omnibus (GEO) database, we analysed a merged expression matrix of pulpitis, aiming to identify biological pathways and diagnostic biomarkers of pulpitis.
methodsBy integrating two datasets (GSE77459 and GSE92681) in the GEO database using the sva and limma packages of R, differentially expressed genes (DEGs) of pulpitis were identified. Then, the DEGs were analysed to identify biological pathways of dental pulp inflammation with Gene Ontology (GO) analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis and Gene Set Enrichment Analysis (GSEA). Protein-protein interaction (PPI) networks and modules were constructed to identify hub genes with the Search Tool for the Retrieval of Interacting Genes/Proteins (STRING) and Cytoscape.
resultsA total of 470 DEGs comprising 394 upregulated and 76 downregulated genes were found in pulpitis tissue. GO analysis revealed that the DEGs were enriched in biological processes related to inflammation, and the enriched pathways in the KEGG pathway analysis were cytokine-cytokine receptor interaction, chemokine signalling pathway and NF-κB signalling pathway. The GSEA results provided further functional annotations, including complement system, IL6/JAK/STAT3 signalling pathway and inflammatory response pathways. According to the degrees of nodes in the PPI network, 10 hub genes were identified, and 8 diagnostic biomarker candidates were screened: PTPRC, CD86, CCL2, IL6, TLR8, MMP9, CXCL8 and ICAM1.
conclusionsWith bioinformatics analysis of merged datasets, biomarker candidates of pulpitis were screened and the findings may be as reference to develop a new method of pulpitis diagnosis.
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