Evidence map›Paper›PMID 33044803›Full record

ArticleCurrent protocols in cell biology2020

Proximity Ligation Assay for Detecting Protein-Protein Interactions and Protein Modifications in Cells and Tissues in Situ.

Marihan Hegazy, Eran Cohen-Barak, Jennifer L Koetsier, Nicole A Najor, Constadina Arvanitis, Eli Sprecher, Kathleen J Green, Lisa M Godsel

Abstract read
In one paragraph

Article in Current protocols in cell biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 92 papers.

0numbers the graph read from it
0cells of the map it votes in
92citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

92 citing papers in PubMed.

  1. Type III intermediate filaments as novel CoAlation targets.Redox report : communications in free radical research · 2026
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  10. Frontotemporal dementia associated CHCHD10Cell death & disease · 2026
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  12. Cylindrical Crystallization of CaInternational journal of molecular sciences · 2026
    Review
  13. c-MYC is an aggregation-prone, amyloidogenic protein.bioRxiv : the preprint server for biology · 2026
    Article
  14. A Potential Novel Molecular Interaction in Bronchiolitis Obliterans Syndrome in Lung Transplantation Patients: The Role of SERPINA3 and Osteoprotegerin.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2026
    Article
  15. Article
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32 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Marihan HegazyDepartment of Pathology, Northwestern University Feinberg School of Medicine, Chicago, Illinois.
Eran Cohen-BarakDepartment of Dermatology, Emek Medical Center, Afula, Israel.
Jennifer L KoetsierDepartment of Pathology, Northwestern University Feinberg School of Medicine, Chicago, Illinois.
Nicole A NajorDepartment of Biology, University of Detroit Mercy, Detroit, Michigan.
Constadina ArvanitisCenter for Advanced Microscopy/Nikon Imaging Center, Northwestern University Feinberg School of Medicine, Chicago, Illinois.
Eli SprecherDepartment of Dermatology, Tel Aviv Medical Center, Tel Aviv, Israel.
Kathleen J GreenDepartment of Pathology, Northwestern University Feinberg School of Medicine, Chicago, Illinois.
Lisa M GodselDepartment of Pathology, Northwestern University Feinberg School of Medicine, Chicago, Illinois.

Funding

Tumor Environment and Metastasis (TEAM) Research ProgramP30CA060553 · NCI · NORTHWESTERN UNIVERSITY AT CHICAGO · PI LEONIDAS C. PLATANIAS · 1993 to 2026
$153.9M
MOLECULAR GENETICS OF PEMPHIGUS FOLIACEUS ANTIGENR01AR041836 · NIAMS · NORTHWESTERN UNIVERSITY AT CHICAGO · PI Kathleen Janee Green · 1993 to 2026
$11.8M
CARCINOGENESIS TRAINING PROGRAMT32CA009560 · NCI · NORTHWESTERN UNIVERSITY AT CHICAGO · PI Kathleen Janee Green · 1986 to 2026
$8.4M
DESMOPLAKIN FUNCTION IN EPIDERMISR01AR043380 · NIAMS · NORTHWESTERN UNIVERSITY AT CHICAGO · PI Kathleen Janee Green · 1996 to 2026
$7.0M
Northwestern University Skin Disease Research Center Resource-based CenterP30AR075049 · NIAMS · NORTHWESTERN UNIVERSITY AT CHICAGO · PI Irina Budunova · 2019 to 2026
$6.6M
The Role of Keratinocytes as Independent ThermosensorsP30AR057216 · NIAMS · NORTHWESTERN UNIVERSITY AT CHICAGO · PI PALLER, AMY S · 2009 to 2018
$6.0M
Desmoplakin Assembly and Function in EpidermisR37AR043380 · NIAMS · NORTHWESTERN UNIVERSITY AT CHICAGO · PI GREEN, KATHLEEN JANEE · 2011 to 2020
$4.6M
Role of Desmoglein 1 in Keratinocyte-Melanocyte Communication and MelanomaR01CA228196 · NCI · NORTHWESTERN UNIVERSITY AT CHICAGO · PI Kathleen Janee Green · 2019 to 2026
$3.7M
NCI NIH HHS P30 CA060553NCI NIH HHS R01 CA228196NCI NIH HHS T32 CA009560NIAMS NIH HHS P30 AR057216NIAMS NIH HHS P30 AR075049NIAMS NIH HHS R01 AR041836NIAMS NIH HHS R01 AR043380NIAMS NIH HHS R37 AR043380
6 · The paper itself

Abstract

Biochemical methods can reveal stable protein-protein interactions occurring within cells, but the ability to observe transient events and to visualize the subcellular localization of protein-protein interactions in cells and tissues in situ provides important additional information. The Proximity Ligation Assay

Indexed as

Organ SpecificityProtein Processing, Post-TranslationalAnimalsAntigensBiological AssayCellsFormaldehydeHumansImaging, Three-DimensionalParaffin EmbeddingProtein Interaction MappingTissue FixationAntigensFormaldehydeDuolink™in situ tissue stainingpost-translational modificationprotein-protein interactionproximity ligation assay®

Identifiers

PMID33044803
PMCPMC8041061

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.