ArticleApplied microbiology and biotechnology2020
A novel Rap-Phr system in Bacillus velezensis NAU-B3 regulates surfactin production and sporulation via interaction with ComA.
Article in Applied microbiology and biotechnology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 34 citations in OpenAlex.
- Molecular mechanisms and applications of antimicrobial secondary metabolites ofApplied and environmental microbiology · 2026Review
- ACR3 as a Key Regulator Antimicrobial Lipopeptide Synthesis and Efflux in Bacillus velezensis HN-1.Molecular plant pathology · 2026Article
- Whole-genome analysis reveals the growth-promoting and biocontrol potential of Bacillus amyloliquefaciens Ba. YN.J3 isolated from Avena Sativa.BMC genomics · 2026Article
- Genomic and transcriptomic analysis of yield enhancement mechanism of lipopeptide iturin W by tryptone supplementation.Microbiology spectrum · 2026Article
- Engineering a PhrC-RapC-SinR quorum sensing molecular switch for dynamic fine-tuning of menaquinone-7 synthesis in Bacillus subtilis.Microbial cell factories · 2025Article
- Biocontrol Mechanism ofBiomolecules · 2025Article
- Multi-omics investigation of high-transglutaminase production mechanisms inFrontiers in microbiology · 2025Article
- Autoinducer-2 relieves soil stress-induced dormancy of Bacillus velezensis by modulating sporulation signaling.NPJ biofilms and microbiomes · 2024Article
- Research advances in the identification of regulatory mechanisms of surfactin production by Bacillus: a review.Microbial cell factories · 2024Review
- degQ associated with the degS/degU two-component system regulates biofilm formation, antimicrobial metabolite production, and biocontrol activity in Bacillus velezensis DMW1.Molecular plant pathology · 2023Article
- A review on surfactin: molecular regulation of biosynthesis.Archives of microbiology · 2023Review
- Transcription Factor Spo0A Regulates the Biosynthesis of Difficidin in Bacillus amyloliquefaciens.Microbiology spectrum · 2023Article
- Evaluation of the Biocontrol Efficiency ofPathogens (Basel, Switzerland) · 2023Article
- Toxicity ofInternational journal of molecular sciences · 2022Article
- γ-Polyglutamic Acid Production, Biocontrol, and Stress Tolerance: Multifunction ofInternational journal of environmental research and public health · 2022Article
- The Role of Pyoluteorin fromInternational journal of molecular sciences · 2022Article
- Article
- Beneficial Effects of Spore-FormingFrontiers in veterinary science · 2022Article
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Authors and funding
13 authors at 4 institutions in 2 countries.
Funding
Abstract
Several quorum sensing systems occurring in Bacillus subtilis, e.g. Rap-Phr systems, were reported to interact with major regulatory proteins, such as ComA, DegU, and Spo0A, in order to regulate competence, sporulation, and synthesis of secondary metabolites. In this study, we characterized a novel Rap-Phr system, RapA4-PhrA4, in Bacillus velezensis NAU-B3. We found that the rapA4 and phrA4 genes were co-transcribed in NAU-B3. When rapA4 was expressed in the heterologous host Bacillus subtilis OKB105, surfactin production and sporulation were severely inhibited. However, when the phrA4 was co-expressed, the RapA4 activity was inhibited. The transcription of the surfactin synthetase srfA gene and sporulation-related genes were also regulated by the RapA4-PhrA4 system. In vitro results obtained from electrophoretic mobility shift assay (EMSA) proved that RapA4 inhibits ComA binding to the promoter of the srfA operon, and the PhrA4 pentapeptide acts as anti-activator of RapA4. We also found that the F24 residue plays a key role in RapA4 function. This study indicated that the novel RapA4-PhrA4 system regulates the surfactin synthesis and sporulation via interaction with ComA, thereby supporting the bacterium to compete and to survive in a hostile environment. KEY POINTS: •Bacillus velezensis NAU-B3 has a novel Rap-Phr quorum sensing system, which does not occur in model strains Bacillus subtilis 168 and B. velezensis FZB42. •RapA4-PhrA4 regulates surfactin production and sporulation. •RapA4-PhrA4 interacts with the ComA protein from ComP/ComA two-component system.
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