Evidence map›Paper›PMID 33043224›Full record

ArticleACS omega2020

Mammalian Expression and

Brian J Grindel, Brian J Engel, Carolyn G Hall, Lindsay E Kelderhouse, Anthony Lucci, Niki M Zacharias, Terry T Takahashi, Steven W Millward

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in ACS omega, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Brian J GrindelDepartment of Cancer Systems Imaging, MD Anderson Cancer Center, Houston, Texas 77030, United States.
Brian J EngelDepartment of Cancer Systems Imaging, MD Anderson Cancer Center, Houston, Texas 77030, United States.
Carolyn G HallDepartment of Breast Surgical Oncology, MD Anderson Cancer Center, Houston, Texas 77030, United States.
Lindsay E KelderhouseDepartment of Cancer Systems Imaging, MD Anderson Cancer Center, Houston, Texas 77030, United States.
Anthony LucciDepartment of Breast Surgical Oncology, MD Anderson Cancer Center, Houston, Texas 77030, United States.
Niki M ZachariasDepartment of Urology, MD Anderson Cancer Center, Houston, Texas 77030, United States.
Terry T TakahashiDepartment of Chemistry, University of Southern California, Los Angeles, California 90089, United States.
Steven W MillwardDepartment of Cancer Systems Imaging, MD Anderson Cancer Center, Houston, Texas 77030, United States.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Directed evolution is a powerful tool for the selection of functional ligands from molecular libraries. Extracellular domains (ECDs) of cell surface receptors are common selection targets for therapeutic and imaging agent development. Unfortunately, these proteins are often post-translationally modified and are therefore unsuitable for expression in bacterial systems. Directional immobilization of these targets is further hampered by the absence of biorthogonal groups for site-specific chemical conjugation. We have developed a nonadherent mammalian expression system for rapid, high-yield expression of biotinylated ECDs. ECDs from EGFR, HER2, and HER3 were site-specifically biotinylated

Identifiers

PMID33043224
PMCPMC7542843

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.