ArticleMolecular oral microbiology2021
Multiple factors are involved in regulation of extracellular membrane vesicle biogenesis in Streptococcus mutans.
Article in Molecular oral microbiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 23 citations in OpenAlex.
- Quorum sensing rewires membrane vesicle protein cargo to promote antibiotic persistence inJournal of bacteriology · 2026Article
- From biogenesis to host modulation: the expanding biology of oral streptococcal membrane vesicles.FEMS microbiology reviews · 2026Review
- Characterization of a unique phosphopantetheinyl transferase (PPTase) in the fish pathogen Pseudomonas plecoglossicida.PloS one · 2026Article
- Recent advances in membrane vesicles ofJournal of oral microbiology · 2026Review
- DNA adenine methylation influences gene expression and biofilm formation inApplied and environmental microbiology · 2025Article
- Streptococcus mutans-derived extracellular vesicles promote skin wound healing via tRNA cargo.Journal of nanobiotechnology · 2025Article
- Decoding Adenine DNA Methylation Effects in Streptococcus Mutans: Insights Into Self-DNA Protection and Autoaggregation.Molecular oral microbiology · 2025Article
- A brief review of membrane vesicles fromFrontiers in microbiology · 2025Review
- Bacterial extracellular vesicle: A non-negligible component in biofilm life cycle and challenges in biofilm treatments.Biofilm · 2024Review
- Bile promotes Lactobacillus johnsonii N6.2 extracellular vesicle production with conserved immunomodulatory properties.Scientific reports · 2024Article
- Prokaryotic microvesicles Ortholog of eukaryotic extracellular vesicles in biomedical fields.Cell communication and signaling : CCS · 2024Review
- Engineered bacterial outer membrane vesicles: a versatile bacteria-based weapon against gastrointestinal tumors.Theranostics · 2024Review
- Membrane vesicles released by Lacticaseibacillus casei BL23 inhibit the biofilm formation of Salmonella Enteritidis.Scientific reports · 2023Article
- [Research Updates: Cariogenic Mechanism ofSichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition · 2022Review
- Article
- Complete Genome Sequence of Streptococcus mutans 27-3, an Active Extracellular Membrane Vesicle Producer.Microbiology resource announcements · 2021Article
- Engineered Remolding and Application of Bacterial Membrane Vesicles.Frontiers in microbiology · 2021Review
- The Impacts of Sortase A and the 4'-Phosphopantetheinyl Transferase Homolog Sfp onFrontiers in microbiology · 2020Article
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
Streptococcus mutans, a major etiological agent of human dental caries, produces membrane vesicles (MVs) that contain protein and extracellular DNA. In this study, functional genomics, along with in vitro biofilm models, was used to identify factors that regulate MV biogenesis. Our results showed that when added to growth medium, MVs significantly enhanced biofilm formation by S. mutans, especially during growth in sucrose. This effect occurred in the presence and absence of added human saliva. Functional genomics revealed several genes, including sfp, which have a major effect on S. mutans MVs. In Bacillus sp. sfp encodes a 4'-phosphopantetheinyl transferase that contributes to surfactin biosynthesis and impacts vesiculogenesis. In S. mutans, sfp resides within the TnSmu2 Genomic Island that supports pigment production associated with oxidative stress tolerance. Compared to the UA159 parent, the Δsfp mutant, TW406, demonstrated a 1.74-fold (p < .05) higher MV yield as measured by BCA protein assay. This mutant also displayed increased susceptibility to low pH and oxidative stressors, as demonstrated by acid killing and hydrogen peroxide challenge assays. Deficiency of bacA, a putative surfactin synthetase homolog within TnSmu2, and especially dac and pdeA that encode a di-adenylyl cyclase and a phosphodiesterase, respectively, also significantly increased MV yield (p < .05). However, elimination of bacA2, a bacitracin synthetase homolog, resulted in a >1.5-fold (p < .05) reduction of MV yield. These results demonstrate that S. mutans MV properties are regulated by genes within and outside of the TnSmu2 island, and that as a major particulate component of the biofilm matrix, MVs significantly influence biofilm formation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.