Evidence map›Paper›PMID 33040266›Full record

ArticleMolecular biology reports2020

Fingerprint identification of white clover cultivars based on SSR molecular markers.

Sainan Ma, Chongyang Han, Jie Zhou, Ruchang Hu, Xing Jiang, Feifei Wu, Ke Tian, Gang Nie, Xinquan Zhang

Abstract read
PubMed Publisher
In one paragraph

Article in Molecular biology reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
3.5field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 25 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Sainan MaDepartment of Grassland Science, Animal Science and Technology College, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Chongyang HanDepartment of Grassland Science, Animal Science and Technology College, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Jie ZhouDepartment of Grassland Science, Animal Science and Technology College, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Ruchang HuDepartment of Grassland Science, Animal Science and Technology College, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Xing JiangDepartment of Grassland Science, Animal Science and Technology College, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Feifei WuDepartment of Grassland Science, Animal Science and Technology College, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Ke TianDepartment of Grassland Science, Animal Science and Technology College, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Gang NieDepartment of Grassland Science, Animal Science and Technology College, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. nieg17@sicau.edu.cn.
Xinquan ZhangDepartment of Grassland Science, Animal Science and Technology College, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. zhangxq@sicau.edu.cn.ORCID http://orcid.org/0000-0002-1433-9510
Sichuan Agricultural University · CN

Funding

Modern Agro-industry Technology Research System CARS-34the Grassland Basic Resources Investigation Research in China 2017FY100602
6 · The paper itself

Abstract

White clover (Trifolium repens L.) is an important perennial legume forage with high productivity and quality. To strengthen the basic research on the genetic characteristics, fingerprint identification and adaptability of white clover germplasm resources, Simple sequence repeat (SSR) molecular markers were applied to 10 white clover cultivars to assess the genetic diversity and related lines of white clover at the molecular level in order to lay a theoretical foundation for the selection of high-quality seeds and cultivars of white clover. A total of 120 different bands were amplified by 29 pairs of SSR primers with good polymorphism, of which 103 (89.5%) were polymorphic. Meanwhile, the PIC of each primer was 0.181-0.588, with an average of 0.329. Analysis of molecular variance revealed that 57% of the genetic variation occurred within cultivars and 43% occurred among cultivars. The results of cluster analysis and the principal coordinate analysis revealed that the parental relationships of the 10 cultivars, with the 'Purple' cultivar very distantly related to the other 9 cultivars and the closest parental relationship between 'Ladino' and 'Sulky'. The fingerprints constructed by three representative primers (gtrs679, gtrs319, and gtrs678) have a strong identification ability. In summary, the SSR markers had good polymorphism and could be used for DNA fingerprint analysis of white clover cultivars.

Indexed as

Genetic VariationCluster AnalysisDNA FingerprintingDNA, PlantMicrosatellite RepeatsPolymerase Chain ReactionSpecies SpecificityTrifoliumDNA, PlantDNA fingerprintingGenetic diversitySSRTrifolium repens L.

Identifiers

PMID33040266
OpenAlexW3092622198

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.