Evidence map›Paper›PMID 33037626›Full record

ArticleFEBS letters2020

Regulation of human sphingomyelin synthase 1 translation through its 5'-untranslated region.

Foysal Daian, Brecken Shenandoah Esper, Navid Ashrafi, Gui-Qin Yu, Gabriella Luciano, Sitapriya Moorthi, Chiara Luberto

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in FEBS letters, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.2field-weighted citation impact, top 49% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. Sphingomyelin Synthase Family and Phospholipase Cs.Advances in experimental medicine and biology · 2022
    Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Foysal DaianRenaissance School of Medicine, Stony Brook University, NY, USA.
Brecken Shenandoah EsperRenaissance School of Medicine, Stony Brook University, NY, USA.
Navid AshrafiDepartment of Physiology and Biophysics, Stony Brook University, NY, USA.
Gui-Qin YuDepartment of Physiology and Biophysics, Stony Brook University, NY, USA.
Gabriella LucianoDepartment of Physiology and Biophysics, Stony Brook University, NY, USA.
Sitapriya MoorthiDepartment of Physiology and Biophysics, Stony Brook University, NY, USA.
Chiara LubertoDepartment of Physiology and Biophysics, Stony Brook University, NY, USA.ORCID 0000-0002-0171-2398
Stony Brook University · USStony Brook School · US

Funding

Sphingolipids in Cancer Therapy and AngiogenesisP01CA097132 · NCI · STATE UNIVERSITY NEW YORK STONY BROOK · PI YUSUF AWNI HANNUN · 2003 to 2026
$30.8M
NCI NIH HHS P01 CA097132NCI NIH HHS P01-CA097132
6 · The paper itself

Abstract

Bcr-abl1 oncogene causes a shift in the transcription start site of the SMS1 gene (SGMS1) encoding the sphingomyelin (SM) synthesizing enzyme, sphingomyelin synthase 1 (SMS1). This results in an mRNA with a significantly shorter 5'-UTR, called 7-SGMS1, which is translated more efficiently than another transcript (IIb-SGMS1) with a longer 5'UTR in Bcr-abl1-positive cells. Here, we determine the effects of these alternative 5'UTRs on SMS1 translation and investigate the key features underlying such regulation. First, the presence of the longer IIb 5'UTR is sufficient to greatly impair translation of a reporter gene. Deletion of the upstream open reading frame (-164 nt) or of the predicted stem-loops in the 5'UTR of IIb-SGMS1 has minimal effects on SGMS1 translation. Conversely, deletion of nucleotides -310 to -132 enhanced transcription of IIb-SGMS1 to reach that of 7-SGMS1. We thus suggest that regulatory features within nucleotides -310 and -132 modulate IIb-SGMS1 translation efficiency.

Indexed as

5' Untranslated RegionsFusion Proteins, bcr-ablGene Expression Regulation, NeoplasticHeLa CellsHumansMembrane ProteinsNerve Tissue ProteinsNucleic Acid ConformationProtein BiosynthesisRNA, MessengerTranscription Initiation SiteTransferases (Other Substituted Phosphate Groups)5' Untranslated RegionsBCR-ABL1 fusion protein, humanFusion Proteins, bcr-ablMembrane ProteinsNerve Tissue ProteinsRNA, MessengerSGMS1 protein, humanTransferases (Other Substituted Phosphate Groups)5′ UTRmRNA secondary structuresSGMS1sphingomyelin synthase 1translational regulation

Identifiers

PMID33037626
PMCPMC7756225
OpenAlexW3092507649

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.