ArticleProceedings of the National Academy of Sciences of the United States of America2020
In vivo mRNA display enables large-scale proteomics by next generation sequencing.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 13 citations in OpenAlex.
- Dosa: A method to covalently barcode proteins for high-throughput biochemistry.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Dosa: A method to covalently barcode proteins for high throughput biochemistry.bioRxiv : the preprint server for biology · 2025Article
- Multimodal Omics Approaches to Aging and Age-Related Diseases.Phenomics (Cham, Switzerland) · 2024Review
- Article
- Assembly of Long-Adapter Single-Strand Oligonucleotide (LASSO) Probes for Massively Parallel Capture of Kilobase Size DNA Targets.Current protocols · 2021Article
- Applications of single-cell genomics and computational strategies to study common disease and population-level variation.Genome research · 2021Article
- Multiscale plant modeling: from genome to phenome and beyond.Emerging topics in life sciences · 2021Review
- Nucleic-acid sequencing of proteomes.Nature reviews. Genetics · 2021Article
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3 authors at 1 institution in 1 country.
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Abstract
Large-scale proteomic methods are essential for the functional characterization of proteins in their native cellular context. However, proteomics has lagged far behind genomic approaches in scalability, standardization, and cost. Here, we introduce in vivo mRNA display, a technology that converts a variety of proteomics applications into a DNA sequencing problem. In vivo-expressed proteins are coupled with their encoding messenger RNAs (mRNAs) via a high-affinity stem-loop RNA binding domain interaction, enabling high-throughput identification of proteins with high sensitivity and specificity by next generation DNA sequencing. We have generated a high-coverage in vivo mRNA display library of the
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Registered trials
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