ArticleFoods (Basel, Switzerland)2020
The Influence of Peptidases in Intestinal Brush Border Membranes on the Absorption of Oligopeptides from Whey Protein Hydrolysate: An Ex Vivo Study Using an Ussing Chamber.
Article in Foods (Basel, Switzerland), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Antihypertensive Peptides and Hydrolysates Derived from Plant Proteins and Their Bioavailability.Foods (Basel, Switzerland) · 2026Review
- Hydrogel-Based Therapeutic Strategies for Post-Cholecystectomy NAFLD: Targeting Bile Acid Signaling, Gut Microbiota, Inflammation, and Hepatic Fibrosis.Gels (Basel, Switzerland) · 2026Review
- Free amino acid-rich egg yolk protein hydrolysate promotes osteogenesis of MC3T3-E1 cells association withFrontiers in nutrition · 2026Article
- The combination of dietary active dry yeast andAnimal nutrition (Zhongguo xu mu shou yi xue hui) · 2025Article
- Effects of a Capsaicin-Based Phytogenic Solution on Intestinal Permeability, Serum Amino Acid Concentrations, and Digestibility in Heat-Stressed Growing Pigs.Animals : an open access journal from MDPI · 2025Article
- Algorithm and ninhydrin method allow for measurement of the postprandial appearance of peptides in blood.Scientific reports · 2025Article
- Quantification of Peptides in Food Hydrolysate fromFoods (Basel, Switzerland) · 2025Article
- A ComprehensiveJournal of medicinal chemistry · 2024Article
- In vitro gastrointestinal digestion methods of carbohydrate-rich foods.Food science & nutrition · 2024Review
- The Current and Promising Oral Delivery Methods for Protein- and Peptide-Based Drugs.International journal of molecular sciences · 2024Review
- Modified throughput ninhydrin method for the qualitative assessment of dietary protein absorption in pig plasma.Biology methods & protocols · 2024Article
- Enhancement of Oral Bioavailability of Protein and Peptide by Polysaccharide-based Nanoparticles.Protein and peptide letters · 2024Review
- A Shared Perspective onJournal of agricultural and food chemistry · 2023Review
- Peptidomics Study of Plant-Based Meat Analogs as a Source of Bioactive Peptides.Foods (Basel, Switzerland) · 2023Article
- Hypoxia-Driven Changes in a Human Intestinal Organoid Model and the Protective Effects of Hydrolyzed Whey.Nutrients · 2023Article
- Does sourdough bread provide clinically relevant health benefits?Frontiers in nutrition · 2023Review
- Article
- Antioxidant Potential of the Sweet Whey-Based BeverageAntioxidants (Basel, Switzerland) · 2022Article
- Article
- Manufacture of Whey Protein Hydrolysates Using Plant Enzymes: Effect of Processing Conditions and Simulated Gastrointestinal Digestion on Angiotensin-I-Converting Enzyme (ACE) Inhibitory Activity.Foods (Basel, Switzerland) · 2022Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
For many years, it was believed that only amino acids, dipeptides, and tripeptides could be absorbed and thus reach the bloodstream. Nowadays, the bioavailability of oligopeptides is also considered possible, leading to new research. This pilot study investigates the activity of brush border enzymes on undigested whey protein hydrolysate (WPH) and on simulated intestinal digested (ID) whey hydrolysate and the subsequent absorption of resultant peptides through the proximal jejunum of a 7-week old piglet setup in an Ussing chamber model. Amongst all samples taken, 884 oligopeptides were identified. The brush border peptidase activity was intense in the first 10 min of the experiment, producing several new peptides in the apical compartment. With respect to the ID substrate, 286 peptides were detected in the basolateral compartment after 120 min of enzyme activity, originating from β-lactoglobulin (60%) and β-casein (20%). Nevertheless, only 0.6 to 3.35% of any specific peptide could pass through the epithelial barrier and thus reach the basolateral compartment. This study demonstrates transepithelial jejunum absorption of whey oligopeptides in an ex vivo model. It also confirmed the proteolytic activity of brush border enzymes on these oligopeptides, giving birth to a myriad of new bioactive peptides available for absorption.
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Registered trials
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