ArticleInternational journal of molecular sciences2020
Genome-Wide Development and Validation of Cost-Effective KASP Marker Assays for Genetic Dissection of Heat Stress Tolerance in Maize.
Article in International journal of molecular sciences, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 41 citations in OpenAlex.
- Multi-model GWAS reveals key loci for leaf angle in maize inbred lines from southeast China.BMC plant biology · 2026Article
- Integrated BSA-Seq and RNA-Seq reveals genes associated with salt tolerance at the germination stage in foxtail millet (Setaria italica (L.) P. Beauv.).BMC plant biology · 2026Article
- Development and validation of KASP markers for powdery mildew resistance in Korean naked barley using QTL-seq.Molecular breeding : new strategies in plant improvement · 2026Article
- Genome-Wide Association Study Dissects the Genetic Architecture of Pericarp Traits in Fresh-Eating Maize.Plants (Basel, Switzerland) · 2025Article
- Development and application of SNP markers to discriminate Korean Perilla (Perilla frutescens) varieties using genomic sequence variations.Scientific reports · 2025Article
- Development and Validation of Multi-Locus GWAS-Based KASP Markers for MaizePlants (Basel, Switzerland) · 2025Article
- Heat stress-induced sterility in oilseed crops: a focus on Brassica napus.Molecular biology reports · 2025Review
- Genome-wide association analysis and KASP markers development for protein quality traits in winter wheat.BMC plant biology · 2025Article
- Article
- Thousands of trait-specific KASP markers designed for diverse breeding applications in rice (Oryza sativa).G3 (Bethesda, Md.) · 2025Article
- The complex transcriptional regulation of heat stress response in maize.Stress biology · 2024Review
- KASP: a high-throughput genotyping system and its applications in major crop plants for biotic and abiotic stress tolerance.Molecular biology reports · 2024Review
- Optimization of In Vitro Embryo Rescue and Development of a Kompetitive Allele-Specific PCR (KASP) Marker Related to Stenospermocarpic Seedlessness in Grape (International journal of molecular sciences · 2023Article
- High-temperature stress in crops: male sterility, yield loss and potential remedy approaches.Plant biotechnology journal · 2023Review
- Detecting Cotton Leaf Curl Virus Resistance Quantitative Trait Loci inPlants (Basel, Switzerland) · 2023Article
- Transcriptional dynamics of maize leaves, pollens and ovules to gain insights into heat stress-related responses.Frontiers in plant science · 2023Article
- 1-nonene plays an important role in the response of maize-aphid-ladybird tritrophic interactions to nitrogen.Frontiers in plant science · 2023Article
- Article
- Identification of accession-specific variants and development of KASP markers for assessing the genetic makeup of Brassica rapa seeds.BMC genomics · 2022Article
- Heat Stress-Mediated Constraints in Maize (Frontiers in plant science · 2022Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Maize is the third most important cereal crop worldwide. However, its production is vulnerable to heat stress, which is expected to become more and more severe in coming years. Germplasm resilient to heat stress has been identified, but its underlying genetic basis remains poorly understood. Genomic mapping technologies can fill the void, provided robust markers are available to tease apart the genotype-phenotype relationship. In the present investigation, we used data from an RNA-seq experiment to identify single nucleotide polymorphisms (SNPs) between two contrasting lines, LM11 and CML25, sensitive and tolerant to heat stress, respectively. The libraries for RNA-seq were made following heat stress treatment from three separate tissues/organs, comprising the top leaf, ovule, and pollen, all of which are highly vulnerable to damage by heat stress. The single nucleotide variants (SNVs) calling used STAR mapper and GATK caller pipelines in a combined approach to identify highly accurate SNPs between the two lines. A total of 554,423, 410,698, and 596,868 SNVs were discovered between LM11 and CML25 after comparing the transcript sequence reads from the leaf, pollen, and ovule libraries, respectively. Hundreds of these SNPs were then selected to develop into genome-wide Kompetitive Allele-Specific PCR (KASP) markers, which were validated to be robust with a successful SNP conversion rate of 71%. Subsequently, these KASP markers were used to effectively genotype an F
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