Evidence map›Paper›PMID 33035795›Full record

ArticleDNA repair2020

The DNA damage-sensing NER repair factor XPC-RAD23B does not recognize bulky DNA lesions with a missing nucleotide opposite the lesion.

Katie M Feher, Alexander Kolbanovskiy, Alexander Durandin, Yoonjung Shim, Jung-Hyun Min, Yuan Cho Lee, Vladimir Shafirovich, Hong Mu, Suse Broyde, Nicholas E Geacintov

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in DNA repair, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.4field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Katie M FeherChemistry Department, New York University, 100 Washington East, New York, NY, 10003-5180, USA.
Alexander KolbanovskiyChemistry Department, New York University, 100 Washington East, New York, NY, 10003-5180, USA.
Alexander DurandinChemistry Department, New York University, 100 Washington East, New York, NY, 10003-5180, USA.
Yoonjung ShimDepartment of Chemistry & Biochemistry, Baylor University, Waco, TX, 76706, USA.
Jung-Hyun MinDepartment of Chemistry & Biochemistry, Baylor University, Waco, TX, 76706, USA.
Yuan Cho LeeChemistry Department, New York University, 100 Washington East, New York, NY, 10003-5180, USA.
Vladimir ShafirovichChemistry Department, New York University, 100 Washington East, New York, NY, 10003-5180, USA.
Hong MuDepartment of Biology, New York University, 100 Washington Square East, New York, N.Y., 10003-5180, USA.
Suse BroydeDepartment of Biology, New York University, 100 Washington Square East, New York, N.Y., 10003-5180, USA.
Nicholas E GeacintovChemistry Department, New York University, 100 Washington East, New York, NY, 10003-5180, USA. Electronic address: ng1@nyu.edu.
New York University · USBaylor University · US

Funding

Environmental DNA Lesions and Mutagenesis: Molecular Mechanisms of Lesion Recognition for Repair and Polymerase BypassR01ES025987 · NIEHS · NEW YORK UNIVERSITY · PI BROYDE, SUSE · 2016 to 2025
$3.6M
Interplay between DNA repair mechanisms in human cells and extractsR01ES027059 · NIEHS · NEW YORK UNIVERSITY · PI SHAFIROVICH, VLADIMIR · 2016 to 2020
$1.8M
Recognition of Environmental Carcinogen-DNA lesions by NER ProteinsR01ES024050 · NIEHS · NEW YORK UNIVERSITY · PI GEACINTOV, NICHOLAS E · 2014 to 2018
$1.8M
Arylamine DNA adduct recognition in eukaryotic nucleotide excision repairR21ES028384 · NIEHS · UNIVERSITY OF RHODE ISLAND · PI CHO, BONGSUP P, MIN, JUNG-HYUN · 2017 to 2018
$443k
NIEHS NIH HHS R01 ES024050NIEHS NIH HHS R01 ES025987NIEHS NIH HHS R01 ES027059NIEHS NIH HHS R21 ES028384
6 · The paper itself

Abstract

The Nucleotide Excision Repair (NER) mechanism removes a wide spectrum of structurally different lesions that critically depend on the binding of the DNA damage sensing NER factor XPC-RAD23B (XPC) to the lesions. The bulky mutagenic benzo[a]pyrene diol epoxide metabolite-derived cis- and trans-B[a]P-dG lesions (G*) adopt base-displaced intercalative (cis) or minor groove (trans) conformations in fully paired DNA duplexes with the canonical C opposite G* (G*:C duplexes). While XPC has a high affinity for binding to these DNA lesions in fully complementary double-stranded DNA, we show here that deleting only the C in the complementary strand opposite the lesion G* embedded in 50-mer duplexes, fully abrogates XPC binding. Accurate values of XPC dissociation constants (K

Indexed as

DNA Repair7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxideDNADNA AdductsDNA-Binding ProteinsDNA Repair EnzymesHumansKineticsMolecular Dynamics SimulationNucleic Acid ConformationProtein ConformationSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsSubstrate Specificity7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxidebenzo(a)pyrene-7,8-dihydrodiol-9,10-epoxide-DNADNADNA AdductsDNA-Binding ProteinsDNA Repair EnzymesRAD23B protein, humanRad4 protein, S cerevisiaeSaccharomyces cerevisiae ProteinsXPC protein, humanBase sequence effectDNA lesionNER-resistanceNucleotide deletionNucleotide excision repairXPC-Rad23B

Identifiers

PMID33035795
PMCPMC8423485
OpenAlexW3090813642

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.