Evidence map›Paper›PMID 33035616›Full record

ArticleJournal of controlled release : official journal of the Controlled Release Society2021

Flt3-L enhances trans-epithelial migration and antigen presentation of dendritic cells adoptively transferred to genital mucosa.

Jaehyung Park, Hannah Frizzell, Hangyu Zhang, Shijie Cao, Sean M Hughes, Florian Hladik, David M Koelle, Kim A Woodrow

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of controlled release : official journal of the Controlled Release Society, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.1field-weighted citation impact, top 57% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Vaginal delivery of vaccines.Advanced drug delivery reviews · 2021
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 2 countries.

Jaehyung ParkDepartment of Bioengineering, University of Washington, Seattle, WA, USA.
Hannah FrizzellDepartment of Bioengineering, University of Washington, Seattle, WA, USA.
Hangyu ZhangDepartment of Bioengineering, University of Washington, Seattle, WA, USA; School of Biomedical Engineering, Key Laboratory of Integrated Circuit and Biomedical Electronic System, Dalian University of Technology, Dalian, Liaoning Province, China.
Shijie CaoDepartment of Bioengineering, University of Washington, Seattle, WA, USA.
Sean M HughesDepartments of Obstetrics and Gynecology, University of Washington, Seattle, WA, USA.
Florian HladikDepartments of Obstetrics and Gynecology, University of Washington, Seattle, WA, USA; Department of Medicine, University of Washington, Seattle, WA, USA; Vaccine and Infectious Diseases Division, Fred Hutchinson Cancer Research Center, Seattle, WA, USA.
David M KoelleDepartments of Laboratory Medicine, and Global Health, University of Washington, Seattle, WA, USA; Department of Medicine, University of Washington, Seattle, WA, USA; Vaccine and Infectious Diseases Division, Fred Hutchinson Cancer Research Center, Seattle, WA, USA; Translational Research Program, Benaroya Research Institute at Virginia Mason, Seattle, WA, USA.
Kim A WoodrowDepartment of Bioengineering, University of Washington, Seattle, WA, USA. Electronic address: woodrow@uw.edu.
University of Washington · USInfectious Disease Research Institute · USVirginia Mason Medical Center · US

Funding

Systems and carcinogenic impact assessment of topical microbicides on human mucosaR01AI116292 · NIAID · UNIVERSITY OF WASHINGTON · PI HLADIK, FLORIAN · 2015 to 2019
$3.5M
Extracellular vesicles in semen and genital HIV infection and immunity during heroin addiction and methadone or buprenorphine substitution therapyR01DA040386 · NIDA · UNIVERSITY OF WASHINGTON · PI HLADIK, FLORIAN · 2015 to 2019
$2.6M
Nanomaterials for engineering protection in the genital mucosaDP2HD075703 · NICHD · UNIVERSITY OF WASHINGTON · PI WOODROW, KIM A. · 2012 to 2012
$2.3M
NIAID NIH HHS R01 AI116292NICHD NIH HHS DP2 HD075703NIDA NIH HHS R01 DA040386
6 · The paper itself

Abstract

Dendritic cells (DCs) play a critical role in shaping adaptive immunity. Systemic transfer of DCs by intravenous injection has been widely investigated to inform the development of immunogenic DCs for use as cellular therapies. Adoptive transfer of DCs to mucosal sites has been limited but serves as a valuable tool to understand the role of the microenvironment on mucosal DC activation, maturation and antigen presentation. Here, we show that chitosan facilitates transmigration of DCs across the vaginal epithelium in the mouse female reproductive tract (FRT). In addition, ex vivo programming of DCs with fms-related tyrosine kinase 3 ligand (Flt3-L) was found to enhance translocation of intravaginally administered DCs to draining lymph nodes (dLNs) and stimulate in vivo proliferation of both antigen-specific CD4+ and CD8+ T cells (cross-presentation). Mucosal priming with chitosan and DC programming may hold great promise to enhance efficacy of DC-based vaccination to the female genital mucosa.

Indexed as

Antigen PresentationDendritic CellsAnimalsCD8-Positive T-LymphocytesFemaleGenitaliaMiceMice, Inbred C57BLMucous MembraneChitosanDendritic cellDraining lymph nodeMucosal vaccineReproductive tract

Identifiers

PMID33035616
PMCPMC7904624
OpenAlexW3092390745

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.