ArticleNucleic acids research2020
A combinatorial method to isolate short ribozymes from complex ribozyme libraries.
Article in Nucleic acids research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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6 citing papers in PubMed.
- Assembly of catalytic complexes from randomized oligonucleotides.Science advances · 2025Article
- Rapid discovery of functional RNA domains.Nucleic acids research · 2025Article
- Principles ofJournal of the Royal Society, Interface · 2025Article
- Peptide conjugates with polyaromatic hydrocarbons can benefit the activity of catalytic RNAs.Chemical science · 2023Article
- Pushing the Limits of Nucleic Acid Function.Chemistry (Weinheim an der Bergstrasse, Germany) · 2022Review
- Secondary Structure Libraries for Artificial Evolution Experiments.Molecules (Basel, Switzerland) · 2021Article
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2 authors.
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Abstract
In vitro selections are the only known methods to generate catalytic RNAs (ribozymes) that do not exist in nature. Such new ribozymes are used as biochemical tools, or to address questions on early stages of life. In both cases, it is helpful to identify the shortest possible ribozymes since they are easier to deploy as a tool, and because they are more likely to have emerged in a prebiotic environment. One of our previous selection experiments led to a library containing hundreds of different ribozyme clusters that catalyze the triphosphorylation of their 5'-terminus. This selection showed that RNA systems can use the prebiotically plausible molecule cyclic trimetaphosphate as an energy source. From this selected ribozyme library, the shortest ribozyme that was previously identified had a length of 67 nucleotides. Here we describe a combinatorial method to identify short ribozymes from libraries containing many ribozymes. Using this protocol on the library of triphosphorylation ribozymes, we identified a 17-nucleotide sequence motif embedded in a 44-nucleotide pseudoknot structure. The described combinatorial approach can be used to analyze libraries obtained by different in vitro selection experiments.
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