ArticleAnalytical and bioanalytical chemistry2020
Discrimination between pathogenic and non-pathogenic E. coli strains by means of Raman microspectroscopy.
Article in Analytical and bioanalytical chemistry, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 31 citations in OpenAlex.
- Lighting the Path: Raman Spectroscopy's Journey Through the Microbial Maze.Molecules (Basel, Switzerland) · 2024Article
- Illuminating the Tiny World: A Navigation Guide for Proper Raman Studies on Microorganisms.Molecules (Basel, Switzerland) · 2024Review
- Article
- The influence of a probiotic/prebiotic supplement on microbial and metabolic parameters of equine cecal fluid or fecal slurry in vitro.Journal of animal science · 2023Article
- Gut dysbiosis in autoimmune diseases: Association with mortality.Frontiers in cellular and infection microbiology · 2023Review
- Influence of temperature and pH on induction of Shiga toxinFrontiers in microbiology · 2023Article
- Application of Microfluidics for Bacterial Identification.Pharmaceuticals (Basel, Switzerland) · 2022Review
- Article
- Genomic and phenotypic comparison ofVirulence · 2022Article
- Comparison of Different Label-Free Raman Spectroscopy Approaches for the Discrimination of Clinical MRSA and MSSA Isolates.Microbiology spectrum · 2022Article
- Detection of multi-resistant clinical strains of E. coli with Raman spectroscopy.Analytical and bioanalytical chemistry · 2022Article
- Noise Sources and Requirements for Confocal Raman Spectrometers in Biosensor Applications.Sensors (Basel, Switzerland) · 2021Article
- Insights into the metal retention role in the antibacterial behavior of montmorillonite and cellulose tissue-supported copper and silver nanoparticles.RSC advances · 2021Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Bacteria can be harmless commensals, beneficial probiotics, or harmful pathogens. Therefore, mankind is challenged to detect and identify bacteria in order to prevent or treat bacterial infections. Examples are identification of species for treatment of infection in clinics and E. coli cell counting for water quality monitoring. Finally, in some instances, the pathogenicity of a species is of interest. The main strategies to investigate pathogenicity are detection of target genes which encode virulence factors. Another strategy could be based on phenotypic identification. Raman spectroscopy is a promising phenotypic method, which offers high sensitivities and specificities for the identification of bacteria species. In this study, we evaluated whether Raman microspectroscopy could be used to determine the pathogenicity of E. coli strains. We used Raman spectra of seven non-pathogenic and seven pathogenic E. coli strains to train a PCA-SVM model. Then, the obtained model was tested by identifying the pathogenicity of three additional E. coli strains. The pathogenicity of these three strains could be correctly identified with a mean sensitivity of 77%, which is suitable for a fast screening of pathogenicity of single bacterial cells. Graphical abstract.
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