ArticleBMC veterinary research2020
Molecular detection and genetic characterization of Anaplasma marginale and Anaplasma platys-like (Rickettsiales: Anaplasmataceae) in water buffalo from eight provinces of Thailand.
Article in BMC veterinary research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it, 35 citations in OpenAlex.
- Global prevalence and species diversity of tick-borne pathogens in buffaloes worldwide: a systematic review and meta-analysis.Parasites & vectors · 2023Pooled it
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- Intelligent identification of medical and veterinary intracellular protozoa by using self-supervised learning.Parasites & vectors · 2026Article
- Molecular Detections of Hematoparasites in Tabanids (Diptera: Tabanidae) from Northeastern Thailand.Pathogens (Basel, Switzerland) · 2025Article
- Isolation, Molecular Characterization and In Vitro Propagation of anPathogens (Basel, Switzerland) · 2025Article
- Epidemiology and factors associated with the infection of Babesia bigemina, Babesia bovis, and Theileria orientalis in Thale Noi Wetland buffaloes (Bubalus bubalis), Southern Thailand.BMC veterinary research · 2025Article
- Application of molecular detection, phylogenetic analysis, and risk factor evaluation for combatingParasitology · 2025Article
- HemotropicCurrent research in parasitology & vector-borne diseases · 2025Article
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- Anaplasma capra: a new emerging tick-borne zoonotic pathogen.Veterinary research communications · 2024Review
- First report on the molecular detection and genetic diversity ofVeterinary world · 2024Article
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- Molecular surveillance and genetic diversity of Anaplasma spp. in cattle (Bos taurus) and goat (Capra aegagrus hircus) from Hainan island/province, China.BMC veterinary research · 2023Article
- Molecular prevalence ofVeterinary world · 2023Article
- Spotted fever group Rickettsia, Anaplasma and Coxiella-like endosymbiont in Haemaphysalis ticks from mammals in Thailand.Veterinary research communications · 2022Article
- Molecular Epidemiological Investigation of Piroplasms and Anaplasmataceae Bacteria in Egyptian Domestic Animals and Associated Ticks.Pathogens (Basel, Switzerland) · 2022Article
- Molecular Detection of Zoonotic and Non-Zoonotic Pathogens from Wild Boars and Their Ticks in the Corsican Wetlands.Pathogens (Basel, Switzerland) · 2021Article
- Molecular Survey ofVeterinary sciences · 2021Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
backgroundAnaplasmosis, an animal disease caused by rickettsial bacteria in the genus Anaplasma, is of considerable economic importance in livestock animals in many countries worldwide. The objectives of this study were to determine the identity, prevalence, and geographic distribution of Ehrlichia and Anaplasma in naturally infected water buffalo in Thailand using PCR amplification and sequencing of the 16S ribosomal RNA and heat shock protein groEL genes. A total of 456 buffalo blood samples from Thailand were investigated. Species identification and genetic differentiation of intra-population and inter-population with the global isolates were conducted based on nucleotide sequences. Interplay between the infection and host factors was also assessed.
resultsOverall, 41% of water buffalo were found to be infected with rickettsial organisms in the family Anaplasmataceae, but Ehrlichia spp., Neorickettsia spp., and Wolbachia spp. were not found in any of the sequenced samples in this study. Female buffalo were more frequently infected with bacteria in the family Anaplasmataceae than males [71 out of 176 females (40.3%) versus 11 out of 47 males (23.4%)]. The Odds Ratio value indicated that the risk of infection for female buffalo was 2.2-fold higher than that for males (p < 0.05). We detected three haplotypes of A. marginale 16S rRNA gene and they were placed in a clade that was closely related to the A. marginale in buffalo in China; and cattle in Thailand, Uganda, and China. Homology searching of groEL sequences against the GenBank™ database using the BLASTn algorithm revealed that the obtained sequences had a high percentage similarity (98.36-99.62%) to A. platys sequences. The groEL sequences of three A. platys-like isolates were clustered in the same clade as the A. platys from the tick Rhipicephalus microplus in China.
conclusionsOur data showed that the apparently healthy buffalo were naturally infected by bacteria in the family Anaplasmataceae at a relatively high prevalence. We also report the finding of A. platys-like infections in water buffalo in Thailand for the first time. Water buffalo serving as the reservoir host of anaplasmosis is of concern for managing the disease control and prevention in ruminants.
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