Evidence map›Paper›PMID 33030626›Full record

ArticleAMB Express2020

One-step purification of two novel thermotolerant β-1,4-glucosidases from a newly isolated strain of Fusarium chlamydosporum HML278 and their characterization.

Yongling Qin, Qiqian Li, Fengfeng Luo, Yue Fu, Haiyan He

Abstract read
In one paragraph

Article in AMB Express, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Optimisation ofInternational journal of molecular sciences · 2023
    Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Yongling QinCollege of Chemistry and Biological Engineering, Hechi University, 546300, Yizhou, China. qin0718@163.com.ORCID http://orcid.org/0000-0001-8921-5742
Qiqian LiCollege of Chemistry and Biological Engineering, Hechi University, 546300, Yizhou, China.
Fengfeng LuoCollege of Chemistry and Biological Engineering, Hechi University, 546300, Yizhou, China.
Yue FuCollege of Chemistry and Biological Engineering, Hechi University, 546300, Yizhou, China.
Haiyan HeCollege of Chemistry and Biological Engineering, Hechi University, 546300, Yizhou, China. hhy_912@163.com.

Funding

National Natural Science Foundation of China (CN) 31660017
6 · The paper itself

Abstract

A newly identified cellulase-producing Fusarium chlamydosporum HML278 was cultivated under solid-state fermentation of sugarcane bagasse, and two new β-glucosides enzymes (BG FH1, BG FH2) were recovered from fermentation solution by modified non-denaturing active gel electrophoresis and gel filtration chromatography. SDS-PAGE analysis showed that the molecular weight of BG FH1 and BG FH2 was 93 kDa and 52 kDa, respectively, and the enzyme activity was 5.6 U/mg and 11.5 U/mg, respectively. The optimal reaction temperature of the enzymes was 60 ℃, and the enzymes were stable with a temperature lower than 70 ℃. The optimal pH of the purified enzymes was 6.0, and the enzymes were stable between pH 4-10. K

Indexed as

Fusarium chlamydosporum HML278heat-resistantpurificationtransglycosidic activityβ-glucosidase

Identifiers

PMID33030626
PMCPMC7544787

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.