ArticleNature communications2020
One-pot biocatalytic route from cycloalkanes to α,ω-dicarboxylic acids by designed Escherichia coli consortia.
Article in Nature communications, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 88 citations in OpenAlex.
- Biosensor-guided directed evolution of aldehyde dehydrogenase for improved adipic acid production from caprolactone.Journal of biological engineering · 2026Article
- Cooperativity in microbial biotechnology: synthetic consortia as emerging metabolic engineering strategy for sustainable bioproduction.Frontiers in bioengineering and biotechnology · 2026Review
- D-CAPS: an efficient CRISPR-Cas9-based phage defense system forActa biochimica et biophysica Sinica · 2025Article
- Multidimensional Engineering of Escherichia coli for Efficient Adipic Acid Synthesis From Cyclohexane.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- A concise enzyme cascade enables the manufacture of natural and halogenated protoberberine alkaloids.Nature communications · 2025Article
- Modular metabolic engineering of Bacillus amyloliquefaciens for high-level production of green biosurfactant iturin A.Applied microbiology and biotechnology · 2024Article
- Modular assembly of an artificially concise biocatalytic cascade for the manufacture of phenethylisoquinoline alkaloids.Nature communications · 2024Article
- Article
- Efficient production of salvianic acid A from L-dihydroxyphenylalanine through a tri-enzyme cascade.Bioresources and bioprocessing · 2023Article
- Polymers without Petrochemicals: Sustainable Routes to Conventional Monomers.Chemical reviews · 2023Review
- Highly efficient biosynthesis of spermidine from L-homoserine and putrescine using an engineered Escherichia coli with NADPH self-sufficient system.Applied microbiology and biotechnology · 2022Article
- One-pot synthesis of (R)- and (S)-phenylglycinol from bio-based L-phenylalanine by an artificial biocatalytic cascade.Bioresources and bioprocessing · 2021Article
- One-pot synthesis of 6-aminohexanoic acid from cyclohexane using mixed-species cultures.Microbial biotechnology · 2021Article
- One-pot production of butyl butyrate from glucose using a cognate "diamond-shaped" E. coli consortium.Bioresources and bioprocessing · 2021Article
- Coproduction of 5-Aminovalerate and δ-Valerolactam for the Synthesis of Nylon 5 From L-Lysine inFrontiers in bioengineering and biotechnology · 2021Article
- Exploring the Potential of Cytochrome P450 CYP109B1 Catalyzed Regio-and Stereoselective Steroid Hydroxylation.Frontiers in chemistry · 2021Article
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Corrections and comments
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Authors and funding
13 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Aliphatic α,ω-dicarboxylic acids (DCAs) are a class of useful chemicals that are currently produced by energy-intensive, multistage chemical oxidations that are hazardous to the environment. Therefore, the development of environmentally friendly, safe, neutral routes to DCAs is important. We report an in vivo artificially designed biocatalytic cascade process for biotransformation of cycloalkanes to DCAs. To reduce protein expression burden and redox constraints caused by multi-enzyme expression in a single microbe, the biocatalytic pathway is divided into three basic Escherichia coli cell modules. The modules possess either redox-neutral or redox-regeneration systems and are combined to form E. coli consortia for use in biotransformations. The designed consortia of E. coli containing the modules efficiently convert cycloalkanes or cycloalkanols to DCAs without addition of exogenous coenzymes. Thus, this developed biocatalytic process provides a promising alternative to the current industrial process for manufacturing DCAs.
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