ArticleNature communications2020
A fluoride-responsive genetic circuit enables in vivo biofluorination in engineered Pseudomonas putida.
Article in Nature communications, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 93 citations in OpenAlex.
- Adaptive evolution ofJournal of bacteriology · 2026Article
- Fluoride triggers lysis inProceedings of the National Academy of Sciences of the United States of America · 2025Article
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- Engineering coupled consortia-based biosensors for diagnostic.Nature communications · 2025Article
- Engineering a phi15-based expression system for stringent gene expression in Pseudomonas putida.Communications biology · 2025Article
- Biosensor-Guided Engineering of a Baeyer-Villiger Monooxygenase for Aliphatic Ester Production.Chembiochem : a European journal of chemical biology · 2025Article
- A prescription for engineering PFAS biodegradation.The Biochemical journal · 2024Review
- A versatile microbial platform as a tunable whole-cell chemical sensor.Nature communications · 2024Article
- The link between ancient microbial fluoride resistance mechanisms and bioengineering organofluorine degradation or synthesis.Nature communications · 2024Review
- Enzymatic synthesis of mono- and trifluorinated alanine enantiomers expands the scope of fluorine biocatalysis.Communications chemistry · 2024Article
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- RecombinantmBio · 2024Article
- Tunable Nanomaterials of Intracellular Crystallization for In Situ Biolabeling and Biomedical Imaging.Chemical & biomedical imaging · 2023Review
- Genome-Wide Expression Analysis Unravels Fluoroalkane Metabolism inEnvironmental science & technology · 2023Article
- Trace impurities in sodium phosphate influences the physiological activity of Escherichia coli in M9 minimal medium.Scientific reports · 2023Article
- Review
- Core and auxiliary functions of one-carbon metabolism inmSystems · 2023Article
- Assessing the Orthogonality of Phage-Encoded RNA Polymerases for Tailored Synthetic Biology Applications inInternational journal of molecular sciences · 2023Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fluorine is a key element in the synthesis of molecules broadly used in medicine, agriculture and materials. Addition of fluorine to organic structures represents a unique strategy for tuning molecular properties, yet this atom is rarely found in Nature and approaches to integrate fluorometabolites into the biochemistry of living cells are scarce. In this work, synthetic gene circuits for organofluorine biosynthesis are implemented in the platform bacterium Pseudomonas putida. By harnessing fluoride-responsive riboswitches and the orthogonal T7 RNA polymerase, biochemical reactions needed for in vivo biofluorination are wired to the presence of fluoride (i.e. circumventing the need of feeding expensive additives). Biosynthesis of fluoronucleotides and fluorosugars in engineered P. putida is demonstrated with mineral fluoride both as only fluorine source (i.e. substrate of the pathway) and as inducer of the synthetic circuit. This approach expands the chemical landscape of cell factories by providing alternative biosynthetic strategies towards fluorinated building-blocks.
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