ArticleInternational journal for parasitology. Drugs and drug resistance2020
The use of ITS-2 rDNA nemabiome metabarcoding to enhance anthelmintic resistance diagnosis and surveillance of ovine gastrointestinal nematodes.
Article in International journal for parasitology. Drugs and drug resistance, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 1 of them a synthesis that pooled it.
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Who cites it
24 citing papers in PubMed, 1 synthesis or guideline pooled it, 69 citations in OpenAlex.
- Transforming gastrointestinal helminth parasite identification in vertebrate hosts with metabarcoding: a systematic review.Parasites & vectors · 2024Pooled it
- Climate-Sensitive Redistribution of Veterinary Parasites: An Attribution Framework for One Health Surveillance and Control.Biology · 2026Review
- Increase in anthelmintic resistance in sheep flocks in Ireland.Irish veterinary journal · 2026Article
- Effectiveness of eprinomectin, albendazole and their combination therapy against strongyle nematode in dairy goats: A clinical field study using nemabiome-integrated approach in Thailand.Current research in parasitology & vector-borne diseases · 2026Article
- QIIME2 pipeline for ITS2-based nemabiome sequencing in veterinary species and the importance of analysis parameters.Parasites & vectors · 2025Article
- Coprological and Molecular Analyses of Ruminant Farms in Québec, Canada, Show a Variable Efficacy of Ivermectin Against Gastro-Intestinal Nematodes.Pathogens (Basel, Switzerland) · 2025Article
- Nemabiome sequencing reveals seasonal and age associated patterns of strongyle infection and high prevalence ofInternational journal for parasitology. Parasites and wildlife · 2025Article
- Old world camels in Germany: parasitic nematode communities characterized by nemabiome analysis showed reduced anthelmintic efficacy according to the fecal egg count reduction test.Parasites & vectors · 2025Article
- The faecal egg count reduction test: Will identification of larvae to species improve its utility?International journal for parasitology. Drugs and drug resistance · 2025Article
- Species diversity and risk factors of gastrointestinal nematodes in smallholder dairy calves in Kenya.Frontiers in veterinary science · 2025Article
- Molecular analysis of feces reveals gastrointestinal nematodes in reintroduced wild asses of the Negev desert.International journal for parasitology. Parasites and wildlife · 2024Article
- Gastrointestinal nematodes in German outdoor-reared pigs based on faecal egg count and next-generation sequencing nemabiome data.Porcine health management · 2024Article
- Occurrence of gastrointestinal nematodes in lambs in Norway, as assessed by copromicroscopy and droplet digital polymerase chain reaction.Acta veterinaria Scandinavica · 2024Article
- Nemabiome metabarcoding shows a high prevalence of Haemonchus contortus and predominance of Camelostrongylus mentulatus in alpaca herds in the northern UK.Parasitology research · 2024Article
- Interaction between bacterial microbiota and nematode parasite communities in sheep's gastrointestinal tract.PloS one · 2024Article
- Anthelmintic resistance in soil-transmitted helminths: One-Health considerations.Parasitology research · 2023Review
- Integration of ITS-2 rDNA nemabiome metabarcoding with Fecal Egg Count Reduction Testing (FECRT) reveals ivermectin resistance in multiple gastrointestinal nematode species, including hypobiotic Ostertagia ostertagi, in western Canadian beef cattle.International journal for parasitology. Drugs and drug resistance · 2023Article
- High diversity and sharing of strongylid nematodes in humans and great apes co-habiting an unprotected area in Cameroon.PLoS neglected tropical diseases · 2023Article
- Article
- Effect of sainfoin (Parasitology · 2022Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A lack of quantitative information on the species composition of parasite communities present in fecal samples is a major limiting factor for the sensitivity, accuracy and interpretation of the diagnostic tests commonly used to assess anthelmintic efficacy and resistance. In this paper, we investigate the ability of ITS-2 rDNA nemabiome metabarcoding to enhance fecal egg count reduction testing by providing information on the effect of drug treatments on individual parasite species. Application of ITS-2 rDNA nemabiome metabarcoding to fecal samples from ewes from over 90 flocks across western Canada revealed high gastrointestinal nematode infection intensities in many flocks with Haemonchus contortus being the most abundant species followed by Teladorsagia circumcincta and then Trichostrongylus colubriformis. Integration of ITS-2 rDNA nemabiome metabarcoding with pre- and post-treatment fecal egg counting revealed consistently poor efficacy of producer-applied ivermectin and benzimidazole treatments against H. contortus, but much better efficacy against T. circumcincta and T. colubriformis, except for in a small number of flocks. Integration of nemabiome ITS-2 rDNA metabarcoding with Fecal Egg Count Reduction Tests (FECRT), undertaken on farm visits, confirmed that ivermectin and fenbendazole resistance is widespread in H. contortus but is currently less common in T. circumcincta and T. colubriformis in western Canada. FECRT/nemabiome testing did not detect moxidectin resistance in any GIN species but suggested the early emergence of levamisole resistance specifically in T. circumcincta. It also revealed that although poor efficacy to closantel was relatively common, based on total fecal egg counts, this was due to its narrow spectrum of activity rather than the emergence of anthelmintic resistance. This study illustrates the value of ITS-2 rDNA nemabiome metabarcoding to improve fecal egg count resistance testing, perform large-scale anthelmintic resistance surveillance and direct more targeted rational anthelmintic use.
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