Evidence map›Paper›PMID 33025316›Full record

ArticleJournal, genetic engineering & biotechnology2020

Suitability of target region amplified polymorphism (TRAP) markers to discern genetic variability in sweet sorghum.

Yehia A Khidr, Sileshi A Mekuriaw, Adel E Hegazy, Enass Amer

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Article in Journal, genetic engineering & biotechnology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

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2citing papers in PubMed
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3 · Its place in the literature

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2 citing papers in PubMed.

  1. Factors affecting the genetic diversity ofFrontiers in plant science · 2026
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4 · The record

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5 · Who and what money

Authors and funding

4 authors.

Yehia A KhidrDepartment of Plant Biotechnology, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Sadat City, Egypt. yehia.khidr@gebri.usc.edu.eg.ORCID http://orcid.org/0000-0002-9861-1074
Sileshi A MekuriawDepartment of Plant Biotechnology, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Sadat City, Egypt.
Adel E HegazyDepartment of Plant Biotechnology, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Sadat City, Egypt.
Enass AmerDepartment of Plant Biotechnology, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Sadat City, Egypt.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundSweet sorghum is an emerging biofuel candidate crop with multiple benefits as a source of biomass energy. Increase of biomass and sugar productivity and quality is a central goal in its improvement. Target region amplified polymorphism (TRAP) is a polymerase chain reaction (PCR) based functional marker system that can detect genetic diversity in the functional region of target genes. Thirty sweet sorghum genotypes were used to study the potential of 24 pairs of TRAP marker system in assessing genetic diversity with regard to three lignin and three sucrose biosynthesis genes.

resultsA total of 1638 bands were produced out of which 1161 (70.88%) were polymorphic at least at one locus. The average polymorphic information content (PIC), resolving power (RP), marker index (MI), Shannon's diversity index (H), and gene diversity values were 0.32, 8.86, 1.74, 3.25, and 0.329, respectively. Analysis of molecular variance (AMOVA) revealed a highly significant genetic variation both within and among accessions studied (P = 0.01). However, the variation within the population was higher than among the populations (accessions). Bootstrap analysis showed that the number of loci amplified using this marker system is sufficient to estimate the available genetic diversity. The thirty genotypes were categorized into five clusters using a similarity matrix at 0.72 coefficient of similarity. The genotypes were also grouped mostly according to their geographic origin where the Ethiopian and Egyptian genotypes tend to fall in specific clusters. Moreover, the genotypes reflected the same pattern of distribution when ordinated using principal coordinate analysis.

conclusionsIn conclusion, TRAP marker can be used as a powerful tool to study genetic diversity in sweet sorghum.

Indexed as

LigninMolecular markersSucrose

Identifiers

PMID33025316
PMCPMC7538518

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