ArticleMedical molecular morphology2021
Three-dimensional electron microscopy for endothelial glycocalyx observation using Alcian blue with silver enhancement.
Article in Medical molecular morphology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 21 citations in OpenAlex.
- Low-vacuum scanning electron microscopy for informative three-dimensional imaging of cell/tissue architectures and biomedical target localization.Microscopy (Oxford, England) · 2026Review
- Age‑dependent and post‑intraventricular hemorrhage remodeling of the ependymal glycocalyx in mice.Fluids and barriers of the CNS · 2025Article
- KMnONpj imaging · 2024Article
- Structure-preserving fixation allows scanning electron microscopy to reveal biofilm microstructure and interactions with immune cells.Journal of microscopy · 2024Article
- Review
- Fast-track preparation of lung specimens for electron microscope observations of the pulmonary endothelial glycocalyx.Medical molecular morphology · 2023Article
- Gemcitabine alters sialic acid binding of the glycocalyx and induces inflammatory cytokine production in cultured endothelial cells.Medical molecular morphology · 2023Article
- Recent advances in electron microscopy for the diagnosis and research of glomerular diseases.Kidney research and clinical practice · 2023Article
- InvestigatingAccess microbiology · 2023Article
- Visualising the endothelial glycocalyx in dogs.Veterinary journal (London, England : 1997) · 2022Article
- The Effects of Resuscitative Fluid Therapy on the Endothelial Surface Layer.Frontiers in veterinary science · 2021Review
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Glycocalyx (GCX) is a thin layer of negatively charged glycoproteins that covers the vascular endothelial surface and regulates various biological processes. Because of the delicate and fragile properties of this structure, it is difficult to detect GCX morphologically. We established a simple method for a three-dimensional visualization of endothelial GCX using low-vacuum scanning electron microscopy (LVSEM) on formalin-fixed paraffin-embedded (FFPE) sections. Mouse kidney tissue was fixed with 10% buffered formalin containing 1% Alcian blue (ALB) via perfusion and immersion. FFPE sections were observed by light microscopy (LM) and LVSEM, and formalin-fixed epoxy resin-embedded ultrathin sections were observed by transmission electron microscopy (TEM). The endothelial GCX from various levels of kidney blood vessels was stained blue in LM and confirmed as a thin osmiophilic layer in TEM. In LVSEM, the sections stained by periodic acid methenamine silver (PAM) revealed the endothelial GCX as a layer of dense silver-enhanced particles, in both the samples fixed via perfusion and immersion. Correlative light and electron microscopy (CLEM) revealed the fine visible structure of endothelial GCX. This simple method using FFPE samples with ALB will enable the three-dimensional evaluation of endothelial GCX alterations in various human diseases associated with endothelial injury in future studies.
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