Evidence map›Paper›PMID 33025084›Full record

ArticleInternational orthopaedics2021

Macrophages' contribution to ectopic osteogenesis in combination with blood clot and bone substitute: possibility for application in bone regeneration strategies.

Jelena M Živković, Sanja T Stojanović, Marija Đ Vukelić-Nikolić, Milena B Radenković, Jelena G Najdanović, Milan Ćirić, Stevo J Najman

Abstract read
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Article in International orthopaedics, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.6field-weighted citation impact, top 36% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 10 citations in OpenAlex.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Jelena M ŽivkovićDepartment of Biology and Human Genetics, Faculty of Medicine, University of Niš, Niš, 18108, Serbia. jelena.zivkovic.biologija@medfak.ni.ac.rs.ORCID https://orcid.org/0000-0002-4010-5742
Sanja T StojanovićDepartment of Biology and Human Genetics, Faculty of Medicine, University of Niš, Niš, 18108, Serbia.ORCID https://orcid.org/0000-0002-6560-6762
Marija Đ Vukelić-NikolićDepartment of Biology and Human Genetics, Faculty of Medicine, University of Niš, Niš, 18108, Serbia.ORCID https://orcid.org/0000-0002-2405-5140
Milena B RadenkovićDepartment for Cell and Tissue Engineering, Scientific Research Center for Biomedicine, Faculty of Medicine, University of Niš, Niš, 18108, Serbia.ORCID https://orcid.org/0000-0003-0603-0684
Jelena G NajdanovićDepartment of Biology and Human Genetics, Faculty of Medicine, University of Niš, Niš, 18108, Serbia.ORCID https://orcid.org/0000-0003-4178-3913
Milan ĆirićInstitute of Physiology, Faculty of Medicine, University of Niš, Niš, 18108, Serbia.ORCID https://orcid.org/0000-0002-4137-4258
Stevo J NajmanDepartment of Biology and Human Genetics, Faculty of Medicine, University of Niš, Niš, 18108, Serbia.ORCID https://orcid.org/0000-0002-2411-9802
University of Nis · RS

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeGiven the great potential of macrophages in the processes of tissue repair and regeneration, the aim of our study was to examine the contribution that macrophages will have in osteogenic process when combined and implanted with blood clot (BC) and mineral bone substitute (MBS) in mice subcutaneous implantation model.

methodsThree types of implants were constructed and implanted subcutaneously into BALB/c mice: (1) RMBM implants (made of resident tissue macrophages, BC and MBS), (2) BM implants (made of BC and MBS), and (3) M implants (made of MBS only) where the last two served as control implants. One, two, four and eight weeks after implantation implants were explanted, and histochemical, immunohistochemical, and histomorphometric analyses were performed.

resultsIncreased vascularization, particularly pronounced two and four weeks after implantation and pronounced tissue infiltration in eight week term in RMBM implants compared with both other types, likewise the presence of osteoblast-like cells, osteoid-like structures, and more prominent osteopontin and osteocalcin immunoexpression in RMBM implants indicated more pronounced osteogenic process within them.

conclusionOur results suggest that macrophages deserve to be considered as a cell component when constructing implants in bone regenerative medicine strategies to improve bone fracture healing process.

Indexed as

Bone SubstitutesThrombosisAnimalsBone RegenerationMacrophagesMiceMice, Inbred BALB COsteogenesisBone SubstitutesBlood clotEctopic osteogenesisImplantMacrophagesMineral bone substituteSubcutaneous implantation

Identifiers

PMID33025084
OpenAlexW3091943398

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.