Evidence map›Paper›PMID 33024969›Full record

ArticlebioRxiv : the preprint server for biology2020

SARS-CoV-2 D614G Variant Exhibits Enhanced Replication

Yixuan J Hou, Shiho Chiba, Peter Halfmann, Camille Ehre, Makoto Kuroda, Kenneth H Dinnon, Sarah R Leist, Alexandra Schäfer, Noriko Nakajima, Kenta Takahashi and 10 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

20 authors.

Yixuan J HouDepartment of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
Shiho ChibaInfluenza Research Institute, Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin, Madison, WI, USA.
Peter HalfmannInfluenza Research Institute, Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin, Madison, WI, USA.
Camille EhreMarsico Lung Institute, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
Makoto KurodaInfluenza Research Institute, Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin, Madison, WI, USA.
Kenneth H DinnonDepartment of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
Sarah R LeistDepartment of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
Alexandra SchäferDepartment of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
Noriko NakajimaDepartment of Pathology, National Institute of Infectious Diseases, Tokyo, Japan.
Kenta TakahashiDepartment of Pathology, National Institute of Infectious Diseases, Tokyo, Japan.
Rhianna E LeeMarsico Lung Institute, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
Teresa M MascenikMarsico Lung Institute, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
Caitlin E EdwardsDepartment of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
Longping V TseDepartment of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
Richard C BoucherMarsico Lung Institute, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
Scott H RandellMarsico Lung Institute, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
Tadaki SuzukiDepartment of Pathology, National Institute of Infectious Diseases, Tokyo, Japan.
Lisa E GralinskiDepartment of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
Yoshihiro KawaokaDivision of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Tokyo, Japan.
Ralph S BaricDepartment of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.

Funding

Project 3: Serological Interactions with the Mucosal Innate Immune System Regulates COVID-19 Associated Tissue Damage.U54CA260543 · NCI · UNIV OF NORTH CAROLINA CHAPEL HILL · PI BARIC, RALPH S · 2020 to 2024
$9.8M
Mechanisms of MERS-CoV Entry, Cross-species Transmission and PathogenesisR01AI110700 · NIAID · UNIV OF NORTH CAROLINA CHAPEL HILL · PI BARIC, RALPH S, LI, FANG · 2015 to 2024
$7.4M
Understanding Risk of Zoonotic Virus Emergence in EID Hotspots of Southeast AsiaU01AI151797 · NIAID · ECOHEALTH ALLIANCE, INC. · PI DASZAK, PETER · 2020 to 2023
$6.0M
Transmissibility of avian influenza viruses in mammalsR01AI069274 · NIAID · UNIVERSITY OF WISCONSIN-MADISON · PI KAWAOKA, YOSHIHIRO · 2006 to 2016
$5.5M
NCI NIH HHS U54 CA260543NIAID NIH HHS HHSN272201400008CNIAID NIH HHS R01 AI069274NIAID NIH HHS R01 AI110700NIAID NIH HHS U01 AI151797
6 · The paper itself

Abstract

The D614G substitution in the S protein is most prevalent SARS-CoV-2 strain circulating globally, but its effects in viral pathogenesis and transmission remain unclear. We engineered SARS-CoV-2 variants harboring the D614G substitution with or without nanoluciferase. The D614G variant replicates more efficiency in primary human proximal airway epithelial cells and is more fit than wildtype (WT) virus in competition studies. With similar morphology to the WT virion, the D614G virus is also more sensitive to SARS-CoV-2 neutralizing antibodies. Infection of human ACE2 transgenic mice and Syrian hamsters with the WT or D614G viruses produced similar titers in respiratory tissue and pulmonary disease. However, the D614G variant exhibited significantly faster droplet transmission between hamsters than the WT virus, early after infection. Our study demonstrated the SARS-CoV2 D614G substitution enhances infectivity, replication fitness, and early transmission.

Identifiers

PMID33024969
PMCPMC7536872

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.